单 位：
上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室
关键词：
松江鲈(Trachidermus fasciatus);RAPD标记;SCAR标记
摘 要：
为寻找松江鲈(Trachidermus fasciatus)鸭绿江种群(YL)、黄河种群(YR)和富春江种群(FR)间的分子标记,从120条10 bp随机引物中,筛选出多态性引物54条进行RAPD扩增。克隆获得了S2069680 bp、S2082331 bp、S2105782 bp与S2111400 bp等4个清晰的RAPD特异性标记,并成功将RAPD标记S2082331 bp与S2105782 bp转化成SCAR03260 bp和SCAR04710 bp的SCAR标记。经每群体30尾样本验证,发现SCAR03260 bp在YL、YR和FR种群中出现的频率依次为13.33%、93.33%、86.67%,可作为鉴别鸭绿江种群与其它两种群的分子标记;相反,SCAR04710 bp在YL、YR种群的出现频率较高(86.67%、83.33%),而在FR种群的出现频率却很低(16.67%),可作为鉴别富春江种群与其它两种群的分子标记。
译 名：
Two new RAPD- SCAR markers among wild populations of Trachidermus fasciatus
作 者：
LI Qiang;LIU Zhi-zhi;ZENG Zhen;PAN Lian-de;WANG Wan-tong;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Shanghai Ocean University Ministry of Education;
关键词：
Trachidermus fasciatus;;RAPD marker;;SCAR marker.
摘 要：
To search for molecular markers among the Yalu River population( YL),Yellow River population( YR) and Fuchun River population( FR) of Trachidermus fasciatus,fifity- four polymorphic 10- bp random primers were selected from 120 ones and used in the RAPD analysis. In total,four clear specific RAPD markers,S2069 680 bp,S2082 331 bp,S2105 782 bp and S2111 400 bpwere obtained. But only S2082 331 bpand S2105 782 bpwere successfully transformed into SCAR markers( SCAR03260 bpand SCAR04710 bp,respectively). Afterwards,the frequencies of the two SCAR makers were tested in 30 samples for each population. As result,the range of frequency for SCAR03260 bpwas 13. 33%,93. 33% and 86. 67% in YL,YR and FR population respectively. Conversely,the frequencies of SCAR04710 bpin YL and YR populations were 86. 67% and 83. 33% respectively,which were obviously higher than that in FR( 16. 67%). Hence,SCAR03260 bpor SCAR04710 bpcan be used as special molecular marker for the population identification between YL and YR,FR,or between FR and YR,YR,respectively.
