Position: Home > Articles > Streptomycin-resistant Rational Screening for High-yield Neomycin Producing Strain
Biotechnology Bulletin
2014,0
(12)
173-176
链霉素抗性突变理性筛选新霉素高产菌株
作 者：
陈宏;张祝兰;孙菲;张引;周璟明;郑榕
单 位：
福建省微生物研究所福建省新药(微生物)筛选重点实验室
关键词：
费氏链霉菌;新霉素;链霉素抗性筛选;突变
摘 要：
采用化学诱变剂NTG结合链霉素抗性筛选法获得新霉素高产菌株。出发菌株费氏链霉菌(Streptomyces fradiae)FS1109的孢子悬液经不同剂量的化学诱变剂NTG处理后,涂布在含链霉素最小抑制浓度(3μg/m L)的培养基平板上培养,获得大量的链霉素抗性突变株。经影印法初筛和摇瓶发酵复筛,正突变率高于负突变率,获得一株遗传性状稳定的Streptomyces fradiae Str 63菌株,其新霉素生物活性单位比出发菌株提高了50%以上,且C组分较出发菌株的低。
译 名：
Streptomycin-resistant Rational Screening for High-yield Neomycin Producing Strain
作 者：
Chen Hong;Zhang Zhulan;Sun Fei;Zhang Yin;Zhou Jingming;Zheng Rong;Fujian Institute of Microbiology,Fujian Provincial Key Laboratory of Screening for Novel Microbial Products;
关键词：
Streptomyces fradiae;;Neomycin;;Streptomycin resistance screening;;Mutation
摘 要：
NTG mutagenesis and streptomycin resistance screening was applied to screen neomycin high producing strain. A number of streptomycin-resistant(str)mutants were obtained from original strain of Streptomyces fradiae FS1109 treated with three different dosage of NTG under the selection pressure of resistance to streptomycin(the lethal concentration is 3 μg/m L). Streptomycin-resistant mutants strain Str63 was obtained with high neomycin yield by replica plating method and the neomycin productivity could be more than 50% higher and the component of C was lower than that of the original strain in the rotation-flask experiments, the rate of the positive mutation was larger than that of the negative mutation.
相似文章
-
费氏链霉菌(Streptomyces fradiae)FIM-S38胞外产新霉素的发酵条件优化 [周璟明, 陈宏, 张祝兰, 孙菲, 张引, 周剑, 方东升] 生物技术通报 2014,0 (12) 92-96
