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Position: Home > Articles > Identification of species and genera of goats and sheep using low copy number of DNA in fur Heilongjiang Animal Science and Veterinary Medicine 2015 (21) 83-84+288

微量毛皮DNA鉴别山羊及绵羊种属

作  者:
张昌盛;孙美荣;李林海;费静;段冀渊;隋阳华
单  位:
上海出入境检验检疫局;北京自然博物馆
关键词:
毛皮;皮革;DNA;物种鉴定;绵羊;山羊
摘  要:
为了鉴别山羊及绵羊种属,试验从动物毛皮入手,尤其是针对DNA难以提取的皮革制品,改良了DNA的抽提方法,使DNA鉴别法普遍适用于毛皮制品。结果表明:利用山羊、绵羊线粒体基因序列的差异,成功鉴别这2个物种。
译  名:
Identification of species and genera of goats and sheep using low copy number of DNA in fur
作  者:
ZHANG Chang-sheng;SUN Mei-rong;LI Lin-hai;FEI Jing;DUAN Ji-yuan;SUI Yang-hua;Beijing Museum of Natural History;Shanghai Entry-Exit Inspection and Quarantine Bureau;
关键词:
fur;;leather;;DNA;;species identification;;sheep;;goat
摘  要:
To identify species and genera of goats and sheep,the test focused on animal fur,especially on leather products that were difficult to extract DNA. A DNA extraction method was modified,which enabled the DNA identification to be generally applicable to the fur products. The results showed that the two species were successfully identified based on the difference between the mitochondrial gene sequences of goats and sheep.

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