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当前位置: 首页 > 文章 > 利用全自动凯氏定氮仪测定大豆粗蛋白含量的条件研究 安徽农业科学 2019,47 (14) 213-215,254
Position: Home > Articles > Study on the Condition of Determination of Soybean Crude Protein Content by Automatic Kjeldahl Analyzer Journal of Anhui Agricultural Sciences 2019,47 (14) 213-215,254

利用全自动凯氏定氮仪测定大豆粗蛋白含量的条件研究

作  者：
游思亮;王青
单  位：
作物遗传与种质创新国家重点实验室
关键词：
全自动凯氏定氮仪;大豆;粗蛋白含量
摘  要：
[目的]研究多种因素对大豆粗蛋白含量测定的影响,以期获得最优化的试验条件.[方法]利用全自动凯氏定氮仪比较不同称样量、催化剂比例、硫酸用量、消化时间、消化温度条件下测定的大豆粗蛋白含量.[结果]当只改变某一种试验条件的水平时,大豆粗蛋白含量测定结果之间差异较大,有的甚至达到极显著水平.针对大豆样本,较优化的试验条件是称样量0.5 g、催化剂用量比例K2 SO4/CuSO4·5H2O为6/0.2(g/g)、硫酸用量为8 mL、消化时间105 min、消化温度420℃.仪器测定硫酸铵的回收率为100.26%,符合定量的要求.[结论]该研究为其他相关研究提供方法借鉴.
译  名：
Study on the Condition of Determination of Soybean Crude Protein Content by Automatic Kjeldahl Analyzer
作  者：
YOU Si-liang;WANG Qing;Nanjing Agricultural University, State Key Laboratory of Crop Genetics and Germplasm Enhancement;
关键词：
Automatic kjeldahl analyzer;;Soybean;;Crude protein content
摘  要：
[Objective]The research aimed to study the effects of various factors on the determination of soybean crude protein content in order to obtain optimal experimental conditions.[Method]Soybean crude protein content was measured by using automatic kjeldahl analyzer under different conditions, which include sample amount, ratio of catalyst, sulfuric acid amount, digesting time and digesting temperature. [Result]When only the level of a certain test condition was changed, the difference between the determination results of the soybean crude protein content was large, and some even reached a very significant level.For the soybean samples, the optimized experimental conditions were 0.5 g soybean sample, the ratio of catalyst to K_2SO_4/CuSO_4·5 H_2O was 6 g/0.2 g, the amount of sulfuric acid was 8 mL, the digestion time was 105 min, and the digestion temperature was 420 ℃.The recovery of(NH_4)_2SO_4 by using automatic kjeldahl analyzer was 100.26%, which was suitable for quantitative analysis.[Conclusion]This study provides a methodological reference for other related research.

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