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当前位置: 首页 > 文章 > 基于茄子基因组重测序的InDel标记开发及应用 植物遗传资源学报 2019 (5) 1278-1288
Position: Home > Articles > Development and Application of Eggplant InDel Markers Based on Whole Genome Re-sequencing Datasets Journal of Plant Genetic Resources 2019 (5) 1278-1288

基于茄子基因组重测序的InDel标记开发及应用

作  者：
吉康娜;郅俊杰;林丹妮;颜爽爽;田时炳;曹必好;邱正坤
单  位：
广东省蔬菜工程技术研究中心/华南农业大学园艺学院
关键词：
茄子;分子标记;InDel;种质分析;品种纯度鉴定
摘  要：
茄子是我国重要的蔬菜作物,然而相比于其他茄果类蔬菜,分子标记在茄子中的应用较少。本研究基于2份在果实与抗性上存在显著差异的茄子的全基因重测序数据,鉴定出151327个InDel位点,随机选择并设计了180个InDel标记,利用重测序材料及其杂交一代进行有效性鉴定,结果显示:180个InDel标记均可获得PCR扩增产物,其中62个标记具有多态性,多态率为34.7%。基于62个InDel标记聚类分析结果揭示出24份紫红长茄自交系的遗传多样性,其中46个标记表现出多态性,每个标记的Nei′s基因多样性指数在0.0799～0.4991之间,Shannon′s多样性指数分布在0.1732～0.6923之间。基于24个InDel标记聚类分析结果揭示出143份茄子品种的遗传多样性,在遗传相似系数0.65处,143份茄子品种大体可以分为Ⅰ～Ⅶ七大类群。基于2对InDel标记的茄子品种纯度鉴定结果与田间鉴定结果相吻合。
译  名：
Development and Application of Eggplant InDel Markers Based on Whole Genome Re-sequencing Datasets
作  者：
JI Kang-na;ZHI Jun-jie;LIN Dan-ni;YAN Shuang-shuang;TIAN Shi-bing;CAO Bi-hao;QIU Zheng-kun;Guangdong Vegetable Engineering and Technology Research Center/College of Horticulture,South China Agricultural University;The Institute of Vegetable and Flower Research,Chongqing Academy of Agricultural Sciences;
关键词：
eggplant;;molecular marker;;InDel;;germplasm analysis;;purity test
摘  要：
Eggplant(Solanum melongena L.)is one of the important vegetables in China.However,the application of molecular markers in eggplant is much less that other solanaceous fruit vegetables.In this study,151327 of InDels were in silico identified by taking advantage of whole genome re-sequencing of two eggplant lines.The InDel markers targeting 180 InDel loci were developed,which were subjected in tests of two re-sequencing eggplant lines and their derived hybrid line.Out of that,sixty-two(34.7%)of total markers were polymorphic between both varieties.62 out of the 180 InDel markers were then used to investigated the genetic diversity and specificity of 24 red-purple long eggplant lines.Among the 62 InDel markers,46 markers were polymorphic among the eggplant lines.The variation of Nei′s gene diversity index and Shannon′s diversity index was between 0.0799-0.4991 and 0.1732-0.6923,respectively.In addition,24 InDel out of the 46 markers were further used to investigated the genetic diversity and specificity of 143 varieties.By using UPGMA,143 varieties were grouped into seven classes(Ⅰ-Ⅶ)when the genetic similarity coefficient is 0.65.The results from purity test of variety from two InDel(EP-215 and EP-1203)markers matched well with those from field experiments.

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