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Position: Home > Articles > Establishment of Regeneration Clone of Basella rubra Anhui Agricultural Science Bulletin 2007,13 (19) 72-74

落葵嫩茎高效再生体系建立的研究

作  者:
孙晓昕;吕博;高嵩;徐娜;姜长阳
单  位:
辽宁师范大学生命科学学院
关键词:
落葵;组织培养;愈伤组织;无性系
摘  要:
以落葵的嫩茎为外植体,在附加不同浓度的BA、NAA、IAA、2,4-D的MS、1/2MS培养基上进行离体培养,诱导嫩茎形成愈伤组织,并分化形成不定苗,建立起嫩茎高效再生体系。结果表明:MS+BA0.6 mg.L-1+2,4-D1.2mg.L-1是诱导嫩茎愈伤组织培养的理想培养基;MS+BA0.6 mg.L-1+NAA0.1 mg.L-1是愈伤组织和不定芽分化培养的理想培养基;1/2 MS+NAA0.6 mg.L-1是落葵生根试管苗的理想培养基;试管苗移栽、扦插的理想基质是炉灰渣;移栽的试管苗生长旺盛。
译  名:
Establishment of Regeneration Clone of Basella rubra
作  者:
Sun Xiaoxin Lu Bo Gao Song Xu Na Jiang Changyang* (College of Biology Scientific,Liaoning Normal University Dalian 116029,China)
关键词:
Basella rubra;Tissue culture;Callus;Clone
摘  要:
The tender stems of Basella rubra were used as the explant and were cultured MS,1/2MS medium supplemented with BA,NAA,IAA,2,4-D of different concentrations to study bud induction and differertiation.The regeneration clones of Basella rubra were established successfully.The results showed that the medium MS+BA0.6 mg·L-1+2,4-D1.2 mg·L-1 was the best for inducing callus,medium MS+BA0.6 mg·L-1+NAA0.1 mg·L-1 was the most suitable for bud differentiation,medium 1/2 MS+NAA0.6 mg·L-1 was the optimum for rooting.The ideal transplantation matrix for tube plantlets was the furnace ashes.The transplanted plantlets grew vigorously.

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