Position: Home > Articles > Real time RT-PCR for detecting expression of phosphotransferase A(aphA) gene of enteropathogenic E.coli isolated from deer
Chinese Journal of Veterinary Science
2007,27
(5)
683-685
实时荧光定量RT-PCR检测鹿源致病性大肠杆菌aphA基因mRNA表达水平
作 者：
张晶;王全凯;王玮;王丽艳;尹秀玲;韩春田;邓旭明;柳巨雄
单 位：
吉林农业大学动物科技学院;河北北方学院;吉林大学畜牧兽医学院;黑龙江畜牧兽医职业学院
关键词：
aphA基因;荧光定量RT-PCR;耐药大肠杆菌;mRNA;鹿
摘 要：
从临床分离的20株鹿源耐氨基糖苷类药物大肠杆菌中选取2株高度耐药菌,4株中度耐药菌和1株低度耐药菌,利用实时荧光定量RT-PCR方法检测编码磷酸转移酶aphA基因mRNA的表达水平。结果表明,2株高度耐药株Ct值(Cycle threshold,即反应管内荧光信号到达设定域值时所经历的循环数)最小,平均为23.40,但这2株之间Ct值也存在着差异;4株中度耐药株之间差异不大,Ct值平均为27.20;而1株低度耐药株Ct值最大,为31.53。这说明不同耐药程度的7株菌在mRNA表达水平上存在差异,且aphA基因的转录、表达水平与耐药水平成正相关。
译 名：
Real time RT-PCR for detecting expression of phosphotransferase A(aphA) gene of enteropathogenic E.coli isolated from deer
作 者：
ZHANG Jing1,2,WANG Quan-kai3,WANG Wei1,WANG Li-yan4,YIN Xiu-ling5,HAN Chun-tian1,DENG Xu-ming1,LIU Ju-xiong1(1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;2.Qingdao Agricultural University,Qingdao,Shandong 266109,China;3.College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China;4.Heilongjiang Vocational College of Animal Husbandry and Veterinary Science,Shuangcheng,Heilongjiang 150111,China;5.Hebei Northern University,Zhangjiakou,Hebei 075031,China)
关键词：
aphA gene;FQ-RT-PCR;drug-resistance E.coli;mRNA;deer
摘 要：
Two higher drug-resistant stains,4 midrange drug-resistant strains and one lower drug-resistant strains were selected from 20 clinical strains which were resistant to aminoglycosides antibiotics,to detect the expression of phosphotransferase A(aphA) gene by real-time RT-PCR.As result,the cycle thresholds of 2 higher drug-resistant stains were different,to be the smallest;That of 4 midrange drug-resistant strains were similar;And that of one lower drug resistant strain was the highest.It demonstrated that the more different levels of drug-resistant the more different quantification of cDNA,and that expressions of aphA gene were positive correlation with the level of drug-resistant.
导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
