单 位：
国家林业局泡桐研究开发中心;中国林业科学研究院经济林研究开发中心
关键词：
‘风味玫瑰’;自交不亲和;花柱;蛋白质组学;质谱鉴定
摘 要：
采用双向凝胶电泳和质谱检测技术,对自交不亲和的杏李品种‘风味玫瑰’自花及异花授粉(‘风味玫瑰’ב恐龙蛋’)的花柱进行差异性蛋白质组学研究。研究结果表明,自花授粉与异花授粉有79个差异蛋白点,表达量差异在2倍以上的蛋白点有43个,其中自花授粉中表达丰度明显升高的蛋白点有6个,表达丰度明显降低的蛋白点有37个。只有34个蛋白点在质谱分析中成功鉴定,其中5种蛋白质分别为催化调节蛋白、防御/胁迫蛋白、结构蛋白等。这5种蛋白质直接或间接地影响花粉管在花柱中的生长。
译 名：
Analysis on Differences of Protein in Stylar Canal During Early Pollination Associated with Self-incompatible Prunus domestica×armeniaca ‘Fengweimeigui'
作 者：
HE Xing-bo;LEI Li-li;YANG Shao-bin;FU Jian-min;LI Fang-dong;Paulownia R&D Center of China,State Forestry Administration;Non-timber Forestry R&D Center,Chinese Academy of Forestry;
关键词：
Prunus domestica×armeniaca ‘Fengweimeigui';;self-incompatibility;;stigma;;proteomics;;mass spectrum
摘 要：
This study focused on the differences of protein in stylar canal between that from self-incompatible Prunus domestica × armeniaca‘Fengweimeigui'and that from cross-pollination( ACP)( ‘Fengweimeigui' × ‘Konglongdan'). Two dimension difference gel electrophoresis( 2D-DIGE) and mass spectrum( MS) detection techniques were used in this study. The results showed that there were 79 different protein spots in stylar canal between that from self-pollination and that from cross-pollination,and there was a huge difference,more than double,in terms of protein expression among 43 protein spots,including 6 up-expressed and 37 down-expressed in self-pollination stylar canal compared with that in cross-pollination stylar canal. Only 34 protein spots were identified unambiguously through mass spectrometry analysis,including 5 kinds which were catalytic protein,defence / stress protein,and structural protein,respectively.
