Position: Home > Articles > Studies on Fermentation Conditions of Antagonistic Streptomyces tumenensis BPS2 against Fulvia fulva
Chinese Agricultural Science Bulletin
2006,22
(5)
341-345
番茄叶霉病菌拮抗链霉菌BPS2发酵条件的研究
作 者：
曹广丽;陈立梅;徐文静;杜茜;李启云;杨石嶂;董英山;许修宏
单 位：
东北农业大学资源与环境学院 哈尔滨150030;吉林农业大学农学院;吉林省农业科学院;东北农业大学资源与环境学院
关键词：
链霉菌BPS2;番茄叶霉病菌;发酵培养基;发酵条件
摘 要：
对番茄叶霉病菌拮抗链霉菌BPS2(StreptomycestumenensisBPS2)菌株的最适培养基成分和发酵条件进行研究。结果表明,F培养基最适合该菌株的发酵培养。通过正交设计试验得出,F培养基最佳碳氮浓度组合为蔗糖1.0%,玉米粉2.0%,蛋白胨0.3%,豆饼粉3.0%,硫酸铵0.5%;发酵参数在培养温度为28℃,180r/min振荡培养条件下,发酵时间60h,装液量250ml三角瓶装70ml,培养基初始pH值为6,种子液菌龄为22h,种子液以6%的接种量转接时发酵液活性最强。
译 名：
Studies on Fermentation Conditions of Antagonistic Streptomyces tumenensis BPS2 against Fulvia fulva
作 者：
Cao Guangli1,2, Chen Limei1,3, Xu Wenjing1, Du Qian1, Li Qiyun1, Yang Shizhang1, Dong Yingshan1, Xu Xiuhong2 (1Jilin Academy of Agriculture Science, Gongzhuling 136100; 2Resource and Environment of Northeast Agriculture University, Harbin 150030; 3Agriculture College of Jilin Agriculture University, Changchun 130118)
关键词：
Streptomyces tumenensis BPS2 fulvia fulva; Fermentation medium;Fermentation situation
摘 要：
This paper studied the fermentation medium and fermentation conditions of Streptomyces tumenensis BPS2 strain against Fulvia fulva. The results indicated that the most appropriate fermentation medium was F medium. Medium components of carbon and nitrogen were studied by quadrate experiment and the optimum medium included sucrose 1%, Cornmeal 2%, Peptone 0.3%, Soybean powder 3% and Ammonia sulfate 0.5% as well. The optimal fermentation parameters are 28℃, 180 r/min, 60 h, pH 6, 22 hours for strain age and 6% inoculation of seeds culture in 250 ml flask.
相似文章
-
玉米弯孢菌叶斑病菌拮抗链霉菌的分离及鉴定 [陈立梅, 徐文静, 李启云, 薛丽静, 杨石嶂, 董英山, 杨信东] 玉米科学 2006,14 (2) 152-155
