单 位：
河南师范大学生命科学学院;新乡市农业局;河南科技学院资源与环境学院
关键词：
柑橘内生菌;分离;柑橘青霉病;拮抗菌;筛选
摘 要：
柑橘青霉病是柑橘贮藏期的主要病害,为利用拮抗内生菌进行生物防治,本研究选取3个品种柑橘的橘皮,采用研磨液培养法分离纯化得到26株内生细菌。通过对峙培养法,发现5株内生细菌对柑橘青霉病菌有明显拮抗作用,占菌株总数的19.2%。对这5株细菌进行初步鉴定,分属于气芽孢杆菌属(Aerobacillus sp.)、芽孢杆菌属(Bacillus sp.)、气单胞菌属(Aeromonas spp.)、假单胞杆菌属(Pseudomonas spp.)和黄单胞杆菌属(Xanthomonas spp.)5个属。不同的柑橘品种橘皮内的内生拮抗细菌的出现频次不同。抑菌结果表明,培养3天时,H-3抑菌圈直径最大,达到了15.3mm,H-18抑菌圈最小,只有5.6mm;4天时,各抑菌圈直径均有缩小,但缩小幅度不大;5天时H-3拮抗作用仍十分明显,抑菌圈直径为10.3mm。根据H-3的菌体形态特征、生理生化测定及其16S rDNA序列分析,鉴定该菌为枯草芽孢杆菌(Bacillus subtilis),该菌的发现对柑橘青霉病的生物防治有较好的应用价值。
译 名：
Studies on Isolation of Endophytic Bacteria from Citrus Skins and Screening of Antagonistic Strains Against Penicillium italicum
作 者：
Liu Qili 1,Zhang Jianxin 2,Xu Ruifu 1,Duan Changyong 3(1 School of Resources & Environment Science,Henan Institute of Science and Technology,Xinxiang Henan 453003;2 College of Life Science,Henan Normal University,Xinxiang Henan 453007;3 Xinxiang Bureau of Agriculture,Xinxiang Henan 453003)
关键词：
Citrus endophytes;isolation;blue mold of citrus;antagonistic bacteria;screening
摘 要：
Citrus green mildew is the main diseases of citrus in storage period.In order to use antagonist endogenous bacteria for biological control,in this study,26 endophytic strains were isolated from the Citrus skins of three selected varieties using the method of grinding liquid culture.By confrontation culture method,seven endophytic strains had obviously antagonism against Penicillium italicum,accounting for 19.2% of the total.Preliminary identification showed the 5 strains belong to Aerobacillus sp.,Bacillus sp.,Aeromonas spp.,Pseudomonas spp.and Xanthomonas spp.,respectively.The occurrence frequency of antagonistic endophytes in different varieties of Citrus was different.Bacteriostatic results showed that bacteriostatic circle diameter of H-3 was the largest,reached 15.3 mm,and that of H-18 was the smallest,only 5.6 mm after cultivated for 3 days;after 4 days,each bacteriostatic circle diameter were narrow,but narrow range was not too much;after 5 days,antagonistic effects of H-3 was still evident,its bacteriostatic circle diameter was 10.3 mm.By morphologic and physiological characteristics,especially by the analysis of 16S rDNA sequences,H-3 was identified as Bacillus subtilis.The found of the endophytic bacterium of H-3 had good application value on biological control of blue mold of Citrus.
