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Position: Home > Articles > A SUITABLE METHOD FOR EXTRACTING TOTAL RNA FROM RED ALGAE Transactions of Oceanology and Limnology 2012 (4) 70-77

一种适合于红藻的总RNA提取方法

作  者:
李天勇;任磊;周冠;刘翠;迟珊;刘涛
单  位:
中国海洋大学海洋生命学院
关键词:
红藻;RNA提取;RT-PCR;actin
摘  要:
以5种红藻(条斑紫菜(Porphyra yezoensis),日本多管藻(Polysiphonia japonica),长心卡帕藻(Kappaphycus alvarezii),扇形叉枝藻(Gymnogongrus ftabelliformis),带形蜈蚣藻(Grate-loupia turuturu))为实验材料,为了获得高质量的总RNA,比较了Trizol法、试剂盒法、CTAB法和Trizol+纯化柱法。结果显示:Trizol+纯化柱法对5种红藻总RNA的提取均取得了良好的效果。经琼脂糖凝胶电泳检测,所提取的总RNA 28S、18S条带清晰明亮,无降解;A260/A280、A260/A230值均符合要求,表明提取的总RNA质量较好;利用提取的总RNA进行RT-PCR扩增actin基因的保守片段,能够获得约550bp的目的片段,进一步表明Trizol+纯化柱法是适合于红藻总RNA提取的方法。
译  名:
A SUITABLE METHOD FOR EXTRACTING TOTAL RNA FROM RED ALGAE
作  者:
LI Tianyong,REN Lei,ZHOU Guan,LIU Cui,CHI Shan,and LIU Tao(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China)
关键词:
red algae;RNA extraction;RT-PCR;actin
摘  要:
In this research,in order to get high quality RNA,compared Trizol method,EASYspin kit method,CTAB method and Trizol + purification column method through selecting five red algae(Porphyra yezoensis,Polysiphonia japonica,Kappaphycus alvarezii,Gymnogongrus ftabelliformis,Grateloupia turuturu)as experiment material.The results showed that Trizol + purification column method for extracting total RNA of 5 red algae have achieved good results.By agarose gel electrophoresis,the 28S and 18S rRNA bands were bright,clear and without degradation;the A260/280 and A260/230 ratio were meet the requirements,showed that the isolated RNA has better quality.we can obtain the target fragment about 550bp by amplified the conserved fragment of actin gene,further showed that Trizol + purification column method was suitable for the algae total RNA extraction.

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