Licochalcone a Inhibits Glycolytic Phenotype and Induces Apoptosis in Melanoma B16F10 Cells

WANG Yanming;LIU Ying;YAN Xinyan;SI Lingling;GAO Caixia;YU Lina;ZHENG Qiusheng;Key Laboratory of Xinjiang Endemic Phytomedicine Resources/School of Pharmacy,Shihezi University;Binzhou Medical University;College of Life Science,Yantai University;

B16F10;;Licochalcone A;;glycolysis;;apoptosis

To investigate the effects of licochalcone A on the glycolytic phenotype and apoptosis in melanoma B16F10 cells. The proliferation of B16F10 cells induced by Licochalcone A was determined by Sulforhodamine B(SRB) method. The levels of extracellular glucose and lactic acid were detected by chromatometry, and intracellular ATP levels were measured by bioluminescence assay. The cellular morphological changes were observed by Giemsa and Hoechst 33258 staining. The apoptosis rate was determined by Annexin V-FITC/PI assay. The proliferation of B16F10 cells treated with Licochalcone A was effectively inhibited in a concentration and time-dependent manner. A substantial decrease in glucose uptake, extracellular lactic acid and intracellular ATP levels were observed. The typical apoptosis morphology(nuclear condensation and fragmentation) was observed with the increasing concentration of Licochalcone A in B16F10 cells, which was associated with the increasing apoptotic rates.It was concluded that the licochalcone A can inhibit glycolytic phenotype and induce apoptosis in melanoma B16F10 cells.