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Position: Home > Articles > Establishment and application of PCR method for detection of Porcine Circovirus Ⅱ Acta Agriculturae Shanghai 2007,23 (4) 102-104

猪圆环病毒Ⅱ型核酸诊断方法的建立和初步应用

作  者:
高骏;胡建华;孙凤萍;李春华;王英;蒋凤英
单  位:
上海农业科学院畜牧兽医研究所;上海实验动物研究中心
关键词:
猪圆环病毒Ⅱ型;PCV-2;PCR
摘  要:
根据Genebank上已发表的猪圆环病毒Ⅱ型(PCV-2)序列设计1对引物PL1/PL2,通过对反应条件的优化,建立了有效的猪圆环病毒Ⅱ型的PCR诊断方法,并对沪郊1株PCV-2分离毒进行了检测和测序鉴定,结果显示此株分离毒为PCV-2病毒。结果显示:新建立的PCV-2的PCR诊断方法具有快速、准确等优点。
译  名:
Establishment and application of PCR method for detection of Porcine Circovirus Ⅱ
作  者:
GAO Jun1,HU Jian-hua2,SUN Feng-ping1,LI Chun-hua1,WANG Ying1,JIANG Feng-ying1 (1Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;2Shanghai Laboratory Animals Research Centre,Shanghai 200032,China)
关键词:
Porcine circovirusⅡ;PCV-2;PCR
摘  要:
A pair of primers PL1PL2 was designed according to the sequences of PCV-2 strain registered by the genebank.A method of effectively detecting PCV2 was established through optimizing reaction conditions.The application of this method to detecting and sequencing the PCV-2 isolate from Shanghai suburbs showed that established PCR method had such advantages as rapidity and precision.

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