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农业科学与技术(英文)
2008,9
(1)
64-66
Position: Home > Articles > Detection for transcriptional activity of Alternaria tenuissima protein elicitor in yeast two-hybrid system.
Agricultural Science & Technology
2008,9
(1)
64-66
Detection for transcriptional activity of Alternaria tenuissima protein elicitor in yeast two-hybrid system.
作 者：
Liu YanFeng;Qiu DeWen;Zeng HongMei;Yang XiuFen
单 位：
China.; Beijing 100081; CAAS;Institute of Plant Protection
关键词：
complementary DNA;DNA libraries;elicitors;genes;genetic vectors;plant pathogenic fungi;plant pathogens;plasmids;restriction endonucleases;transactivation;transcription;Alternaria tenuissima;fungi
摘 要：
The peaT1 gene fragment (which encodes the protein elicitor extracted from Alternaria tenuissima) was amplified from pGEM-6p-1-peaT1 by PCR, and the recovered target gene was cloned into pLexA vector. After digestion and sequencing, the bait vector pLexA-peaT1 was transformed into yeast strain EGY48 [p8op-lacZ] by PEG/LiAC, and the transcriptional activity of bait vector was detected. The results showed that recombinant bait plasmid pLexA-PEMG1 was constructed for the two bands of recombinant bait plasmid in the agarose gel electrophoresis expected after digesting by restriction endonuclease EcoR I and Xho I. Therefore, the recombinant bait plasmid could be used in the yeast two-hybrid system to screen a cDNA library.
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