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当前位置: 首页 > 文章 > 接种固氮菌对尾叶桉抗青枯病的作用及相关酶活性变化 浙江林业科技 2009,29 (4) 7-10
Position: Home > Articles > Effect of Nitrogen-fixation Bacteria on Resistance of Eucalyptus urophylla to Bacteria Wilt Disease and Changes of Defense Enzymes Activity Journal of Zhejiang Forestry Science and Technology 2009,29 (4) 7-10

接种固氮菌对尾叶桉抗青枯病的作用及相关酶活性变化

作  者：
马海宾;康丽华;江业根;郑翠梅
单  位：
中国林业科学研究院热带林业研究所
关键词：
桉树;联合固氮菌;青枯病;酶活性
摘  要：
为了明确固氮菌提高尾叶桉抗青枯病的机制,采用苗期接种固氮菌与青枯病菌的方法,测定固氮菌对桉树的促生效果,分析可溶性蛋白含量和相关防御酶的活性。结果表明,桉树苗接种联合固氮菌Ko1 2个月后,接菌苗高平均达20.53 cm,比对照苗高增加了25.72%,接种固氮菌能够提高桉树苗木成活率7.34%。接种固氮菌的桉树叶片可溶性蛋白含量可以维持在一定水平,抗病相关的防御酶SOD酶、POD酶和PPO酶活性升高,酶比活力均高于接种青枯病菌和不接种对照桉树叶片酶活力。固氮菌对桉树苗有诱导抗病的作用。
译  名：
Effect of Nitrogen-fixation Bacteria on Resistance of Eucalyptus urophylla to Bacteria Wilt Disease and Changes of Defense Enzymes Activity
作  者：
MA Hai-bin,KANG Li-hua,JIANG Ye-gen,ZHENG Cui-mei(Research Institute of Tropical Forestry,CAF,Guangzhou 510520,Guangdong,China)
关键词：
Eucalyptus urophylla;associative nitrogen-fixation bacteria;bacteria wilt disease;enzyme activity
摘  要：
Effect of associative nitrogen-fixation bacteria on Eucalyptus urophylla growth was determined by inoculation of nitrogen-fixation bacteria and Ralstonia solanacearum on Eu.urophylla seedling.The result showed that seedlings inoculated with nitrogen-fixation bacteria had height of 20.53 cm,25.72% higher than the control 2 months after inoculation.And the survival rate of seedlings inoculated increased 7.34% than the control.Analysis on content of soluble protein and activity of defense enzymes demonstrated that content of soluble protein of seedling leaves maintained in a stable level after inoculated with nitrogen-fixation bacteria.While the activity of SOD,POD and PPO in seedlings inoculated with nitrogen-fixation bacteria was higher than in that inoculated with R.solanacearum and in the control.The results indicated that nitrogen-fixation bacteria induced resistance of Eu.urophylla to bacteria wilt disease.

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