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马缨杜鹃下胚轴组织培养及全息现象的研究

作  者:
周艳;高贵龙;邹天才;黄丽华;周洪英;陈训
单  位:
贵州科学院;贵州省植物园
关键词:
马缨杜鹃;组织培养;下胚轴;全息现象
摘  要:
以马缨杜鹃下胚轴为外植体,用L9(34)正交实验法研究基本培养基、NAA、2-ip对芽器官诱导的影响。以马缨杜鹃下胚轴切断为材料,研究全息现象。结果表明马缨杜鹃下胚轴诱导芽的最佳培养配方为1/4 MS+NAA0.75 mg/L+2-ip2.0 mg/L。马缨杜鹃切段在离体培养时存在全息现象,其不定芽发生的部位及数量、频率的梯度变化都遵循生物全息律,全息律的呈现在一定程度上受外源激素和切断位置的影响。
译  名:
Research on the Tissue Culture and the Holographic Phenomenon of Rhododendron delavayi Franch.Hypocotyl
作  者:
ZHOU Yan1,GAO Gui-long2,ZOU Tian-cai1,HUANG Li-hua2,ZHOU Hong-ying1,CHEN Xun2(1.Guizhou Botanical Garden,Guiyang 550001,China;2.Guizhou Academy of Sciences Guiyang,Guiyang 550001,China)
关键词:
Rhododendron delavayi Franch.;plant tissue culture;hypocotyl;bioholographic phenomenon
摘  要:
Using Rhododendron delavayi Franch hypocotyl as explant,through the L9(34) orthogonal experiment,a study was conducted to study the effect of tissue culture on organs sprouting by basic culture medium、NAA、2-ip.Moreover,researched bioholographic phenomenon by hypocotyl cutting tissue culture.Results showed that the optimal combination of bud differentiation was 1/4 MS+0.75 mg/L NAA+2-ip 2.0 mg/L.It was found that in vitro culture of hypocotyl segments showed holographic phenomenon;the position and the quantity、the frequency gradient of sprouting inducing all conformed to the bioholographic law,the hormone and the position of hypocotyl could effect the bioholographic law to a certain extent.

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