单 位：
国家海洋局第二海洋研究所;农业部渔业生态环境重点开放实验室南海水产研究所;农业部海洋与河口渔业重点开放实验室中国水产科学研究院东海水产研究所
关键词：
废电池浸出液;鲫鱼;单细胞凝胶电泳;DNA损伤
摘 要：
应用单细胞凝胶电泳技术(Single cell gel-electrophoresis SCGE)研究了废电池浸出液对鲫鱼红细胞DNA的损伤作用,共分剂量-效应和时间-效应二部分。采用自制废电池浸出液(废电池浸出液为45节1号松下废电池浸泡于40L自来水中90d得到,其中Cu的浓度为28.11μg·L-1,Zn的浓度为3755.61μg·L-1,Pb的浓度为11.70μg·L-1,Cd的浓度为2.01μg·L-1,Hg的浓度为0.77μg·L-1,As的浓度为2.02μg·L-1。),将废电池浸出液与自来水配成5%、10%、20%、30%的溶液进行试验。剂量-效应试验结果表明,5%、10%、20%和30%的废电池浸出液均能在较短时间(48h)内引起鲫鱼红细胞DNA链断裂,出现拖尾现象,即彗星细胞;且随着时间的延长,彗星细胞的数量、彗尾长度和拖尾率均显著增加;DNA损伤程度,在小于2%的范围内,属于中度损伤,在2%～10%的范围内,属于高度损伤,而在大于10%的范围内,属于重度损伤,呈现明显的剂量-效应关系。时间-效应试验结果表明,随着染毒时间的延长,彗星细胞数量增加明显,其中对照组、6h组与其余各组均有显著性差异(P<0.05),彗尾长度及拖尾率也有显著增加,2h内DNA损伤属于中度损伤,2h以后DNA损伤属于高度损伤,呈现明显的时间-效应关系。
译 名：
Effects of Waste Battery Lixivium on DNA Damage of Carassius auratus Erythrocyte
作 者：
JI Yao1,2,SHEN Ang-lv2,PING Xian-yin2,MA Sheng-wei3,SHEN Xin-qiang2(1.The Second Institute of Oceanography,State Oceanic Administration,Hangzhou 310012,China;2.East China Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Key Lab of Marine and Estuary Fisheries,Ministry of Agriculture;Shanghai 200090,China;3.South China Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Key Lab of Fishery Ecology Environment,Ministry of Agriculture,Guangzhou 510300,China)
关键词：
waste battery lixivium;Carassius auratus;single cell gel-electrophoresis;DNA damage
摘 要：
DNA damage induced by waste battery lixivium in erythrocyte of Carassius auratus was studied using single cell gel-electrophoresis(SCGE) assay through two experiments of dose-effect and time-effect.The lixivium was obtained by immersing 45 pieces of R20 National batteries in 40 liters of water for 90 days.The primary metal ions in the lixivium are Cu,Zn,Pb,Cd,Hg and As,with the concentration 28.11,3 755.61,11.70,2.01,0.77 and 2.02μg·L-1 respectively.The C.auratus were exposed to 0%,5%,10%,20% and 30% of waste battery lixivium for 48 h in the dose-effect groups.The results showed that the average comet cell number,length of DNA migration and comet cell rate in all waste battery lixivium exposed groups were significantly higher than that of the negative control group and increased gradually as the concentration of waste battery lixivium increasing.Moderate,serious and extremely serious DNA damage was induced by the waste battery lixivium of less than 2%,2%～10% and 11%～30% respectively.The C.auratus were exposed to 5% waste battery lixivium for 0,6,12,24,48,96 h,7 and 14 days in the time-effect groups.The average comet cell number,length of DNA migration and comet cell rate in all waste battery lixivium exposed groups were significantly higher than that of the control group(P<0.05),and increased as the exposure time extending.The lixivium induced moderate DNA damage in 2 h and serious DNA damage after 2 h.There existed dose-effect relation and time-effect relation under the test concentrations.Waste battery lixivium could induce DNA damage in erythrocyte of C.auratus,which might be a better gene toxicant index in water environment research.
