Position: Home > Articles > Screening of High-yield DHA-producing Crypthecodium cohnii Mutant
FOOD SCIENCE
2013,34
(17)
230-235
高产DHA寇氏隐甲藻突变株的筛选
作 者：
佘隽;田华;陈涛;何东平
单 位：
中国科学院武汉病毒研究所;武汉轻工大学食品科学与工程学院
关键词：
寇式隐甲藻;60Co-γ射线;DHA
摘 要：
以寇氏隐甲藻ATCC30772(C.cohniiATCC30772)为出发菌株,经60Co-γ射线辐照诱变,根据生长发育、苏丹黑B染色镜检、生物量、含油率和DHA含量等指标,经初筛、复筛,选出最佳突变株2.4k-2A2-5,其平均DHA含量达到39.04%,比出发株提高了3.39%,油脂产量和含油率比出发株分别提高了51.66%和63.37%,其DHA产量为7.14g/L发酵液,比出发株提高了近56.92%。
译 名：
Screening of High-yield DHA-producing Crypthecodium cohnii Mutant
作 者：
SHE Jun;TIAN Hua;CHEN Tao;HE Dong-ping;College of Food Science and Technology, Wuhan Polytechnic University;Hubei Pashun Foods Co. Ltd.;Wuhan Institute of Virology,Chinese Academy of Sciences;
关键词：
Crypthecodium cohnii;;60 Coγ rays;;DHA
摘 要：
The original strain C. cohnii ATCC 30772 was irradiated by 60 Coγ ray. Mutant 2.4k-2A 2-5 was selected as a desired positive mutant according to its growth and development, Sudan black B staining, biomass, oil content and DHA content, following screening and rescreening. The average DHA production was 39.04%, which was 3.39% higher than the original strain. The yield and content of oil produced by this mutant was 51.66% and 63.37%, respectively, which were also higher than those by the original strain. The DHA yield(measured in 7.14 g/L fermented liquid) was increased by 56.92% compared to that produced by the original strain.
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