中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 7种中药材斑蝥COI基因序列的分子系统学研究 西南农业学报 2013,26 (5) 1809-1813
Position: Home > Articles > Study on Molecular Phylogeny of Seven Species of Chinese Blister Beetle Based on COI Gene Sequence Southwest China Journal of Agricultural Sciences 2013,26 (5) 1809-1813

7种中药材斑蝥COI基因序列的分子系统学研究

作  者：
陈仕江;鲁增辉;廖玉凤;黄戈;殷幼平
单  位：
重庆大学生命科学学院;重庆市中药研究院
关键词：
斑蝥;COI;DNA条形码
摘  要：
以我国重要的中药资源芫菁科7种斑蝥为研究对象,分析线粒体COI基因序列,探讨COI基因作为DNA条形码在鉴定药用斑蝥基源方面的可行性。采用MEGA 5.0软件分析了不同种之间的碱基组成、遗传距离,利用邻位相连法(NJ)和最大简约法(MP)分别构建了系统发育树。结果显示,PCR扩增获得长约700 bp,GC含量为37.8%~41.4%的COI基因片段。7种斑蝥种间遗传距离较大,介于0.10~0.23之间。系统进化树显示,不同属各自聚为一支,同属不同物种的个体也形成各自独立的分支,基于COI基因的DNA条形码在识别芫菁物种方面和形态学一致。本研究表明以COI基因作为芫菁DNA条形码进行物种鉴定具有一定的可行性。
译  名：
Study on Molecular Phylogeny of Seven Species of Chinese Blister Beetle Based on COI Gene Sequence
作  者：
CHEN Shi-jiang;LU Zeng-hui;LIAO Yu-feng;HUANG Ge;YIN You-ping;Chongqing Academy of Chinese Materia Medica;School of Life Science,Chongqing University;
关键词：
Chinese blister beetle;;COI;;DNA barcode
摘  要：
In this paper,taken seven Chinese blister beetle species as tested objects,their sequences of mitochondrial COI were analyzed,and the viability of the COI gene for DNA barcoding and species identification were also studied. The DNA base composition and genetic distance among the seven species were calculated by using MEGA 5. 0,and the phylogenetic tree were constructed with Neighbor-Joining(NJ)and Maximum Parsimony(MP) methods. The results showed that a 700 bp gene fragment was obtained by using PCR amplification,which GC contents was in the range from 37. 8 % to 41. 4 %. Genetic distance of the seven Chinese blister beetle ranged from 0. 10 to 0. 23. The seven Chinese blister beetle could be easily divided to different branchs by NJ tree and MP tree. Species identification with COI sequences in Chinese blister beetle supported the taxonomy based on morphological characters. The conclusion of our research suggested that mitochondrial COI gene was a valid DNA barcoding gene for specie identification in Meloidae.

相似文章

导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46