关键词：
多鳞四指马鲅;fAFLP;种质资源;遗传多样性;聚类分析
摘 要：
为了解多鳞四指马(鲅)(Eleutheronema thadinum)的遗传多样性信息,采用荧光标记扩增片段长度多态性技术(fluorescent amplified fragment length polymorphism,fAFLP)对多鳞四指马鲅8个地理群体214个样本进行扩增和遗传结构分析.结果显示:用9对引物组合共扩增位点493条,其中多态性位点为443条,多态性比例为89.86%;8个地理群体观测等位基因数(Na)、有效等位基因数(Ne)、Nei多样性指数(H)、Shannon信息指数(I)平均值分别为1.679 0、1.467 2、0.250 8和0.363 1;群体遗传多样性高低依次为舟山>崇明>启东>东山>广州>湛江>琼海>高雄.应用AMOVA对8个群体的遗传变异来源进行分析得出,总的遗传分化指数Fst为0.237 2,表明23.72%遗传变异来自于群体间.8个地理群体遗传距离(D)在0.021 6~0.194 4之间;相似系数(H)在0.823 4~0.978 6之间,UPGMA聚类得出多鳞四指马鲅群体可划分为4个分支.研究结果可为合理评估和保护多鳞四指马鲅资源、开展遗传育种及开发人工养殖技术等提供基础资料.
译 名：
fAFLP analysis on population genetic diversity of Eleutheronema rhadinum along China coast
关键词：
Eleutheronema rhadinum%fAFLP%germplasm resource%genetic diversity%cluster analysis
摘 要：
Eleutheronema rhadinum is a warm marine fish,mainly distributed in the east and south of China coast.E.rhadinum is a valuable marine species with wide adaptability to salinity,fast growth,delicate and delicious meat,and its meat is rich in inorganic salt minerals such as calcium,phosphorus,iron,iodine and vitamins.At present,reports about E.rhadinum are concentrated in the aspects of taxonomy,resource investigation,physiology and morphology.
Some research has been done on genetic diversity of E.rhadinum populations,but the sampling area is relatively scattered and not representative.In this paper,samples of 214 individuals from eight geographical populations of E.rhadinum were collected and amplified to analyze the genetic construction by fluorescent amplified fragment length polymorphism(fAFLP)labeling technology.With nine primer combinations,a total of 493 bands were generated,including 443 polymorphic,and the percentage of polymorphic bands was 89.86%.The mean values of the observed number of alleles(Na),effective number of alleles(Ne),Nei genetic diversity index(H),and Shannon information index(I)of the eight populations were 1.679 0,1.467 2,0.250 8 and 0.363 1.The order of geographical population genetic diversity was Zhoushan>Chongming>Qidong>Dongshan>Guangzhou>Zhanjiang>Qionghai>Gaoxiong.The AMOVA was used to analyze the genetic variation sources of eight populations,and the total genetic differentiation index(Fst)was 0.237 2,indicating that 23.72%of genetic variation came from inter-population.The genetic distance(D)between eight geographical populations ranged from 0.021 6 to 0.194 4 and the similarity coefficient(H)was between 0.823 4 and 0.978 6.Based on research data,the eight populations of E.rhadinum could be divided into four branches by the UPGMA tree.The Chongming population,Qidong population and Zhoushan population were clustered into one branch,Dongshan population and Guangzhou population were clustered into one branch,Gaoxiong group was clustered into one branch,Zhanjiang group and Qionghai group were clustered into one branch.The genetic diversity of E.rhadinum in the coastal waters of China was analyzed systematically by using fAFLP molecular markers,in order to provide basic research data for the assessment and conservation of wild resources of E.rhadinum,and its genetic breeding and artificial breeding.
