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Position: Home > Articles > Effects of Metals on MnP Expression and Wastewater Degradation Efficiency of Xhhh Strain Soils 2007,39 (3) 448-452

金属元素对Xhhh菌株表达MnP酶及降解污染物的影响

作  者:
赵大勇;于洪峰;潘文扬;孙石磊;张徐祥;朱程军;肖琳;崔益斌;程树培
单  位:
南京大学污染控制与资源化研究国家重点实验室;中国矿业大学环境与测绘学院
关键词:
制药废水;金属元素;生物降解;MnP;SDR;土壤修复
摘  要:
测定分析了制药废水中Mn2+、Cu2+、Zn2+和Se4+4种金属元素离子水平对跨界原生质体融合(IKPF)特效菌株Xhhh表达锰过氧化物酶(MnP)水平及降解有机污染物效率的影响。结果表明,影响MnP表达水平的金属元素离子顺序为Mn2+>Se4+>Zn2+>Cu2+。在MnP水平与生物比降解率(SDR)之间,存在极显著的正相关性(P<0.01)。研究结果为应用IKPF-Xhhh特效菌株处理制药废水的工艺设计提供了重要的分子生物学参数。本文还对Xhhh在土壤修复中的应用前景进行了分析讨论。
译  名:
Effects of Metals on MnP Expression and Wastewater Degradation Efficiency of Xhhh Strain
作  者:
ZHAO Da-yong1,YU Hong-feng2,PAN Wen-yang1,SUN Shi-lei1,ZHANG Xu-xiang1,ZHU Cheng-jun1,XIAO Lin1,CUI Yi-bin1,CHENG Shu-pei1 ( 1 State Key Laboratory of Pollution Control and Resource Reuse,Nanjing University,Nanjing 210093,China; 2 School of Environment and Survey,Mining University of China,Xuzhou,Jiangsu 221008,China)
关键词:
Pharmaceutical wastewater,Metals,Biodegradation,MnP,SDR,Soil bioremediation
摘  要:
Effects of 4 metal ions,Mn2+,Cu2+,Zn2+ and Se4+,were determined on level of manganese peroxidase (MnP) expression and degradation efficiency of Strain Xhhh,an inter-kingdom protoplast fusant (IKPF) was constructed for degrading organic pollutants in wastewater from a pharmaceutical plant. Results showed that in terms of specific activity (MnP-SA) level,the four metals were in the order of Mn2+>Se4+>Zn2+ >Cu2+. A significantly positive correlation (P<0.01) was observed between the MnP-SA level and the specific degradation rate (SDR). The findings provided some important molecular biological parameters that were useful in designing wastewater treatment technology using the IKPF-Xhhh strain. The prospect of application of Strain Xhhh to soil bioremediation was also discussed.

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