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当前位置: 首页 > 文章 > 不同保存方法对石榴成熟叶片基因组DNA提取效果的影响 北方园艺 2016 (8) 100-103
Position: Home > Articles > Effect of Different Preservation Methods on Genomic DNA Extraction of Pomegranate Northern Horticulture 2016 (8) 100-103

不同保存方法对石榴成熟叶片基因组DNA提取效果的影响

作  者：
陈芸;高原青;王继莲;马刘峰
单  位：
喀什大学生命与地理科学学院
关键词：
石榴;保存方法;DNA提取
摘  要：
以石榴(Punica granatum L.)成熟叶片为试材,设置6种保存方法,采用改良的CTAB法提取石榴基因组DNA,研究了不同保存方法对石榴叶片基因组DNA提取效果的影响。结果表明:采用改良的CTAB法,新鲜叶片、-70℃保存6个月、4℃保存5d的样品均能得到浓度较高的DNA;-20℃保存7d和14d的样品均能得到完整DNA,但浓度略低;硅胶干燥常温保存5个月的样品用同样的方法未能提取到DNA,在对提取方法进行优化后,可提取纯度较高的基因组DNA,但其浓度仅约为鲜叶的50%。上述方法保存的样品提取的基因组DNA均能得到清晰、稳定的SRAP-PCR扩增图谱。
译  名：
Effect of Different Preservation Methods on Genomic DNA Extraction of Pomegranate
作  者：
CHEN Yun;GAO Yuanqing;WANG Jilian;MA Liufeng;College of Biology and Geography Science,Kashgar University;The Key Laboratory of Ecology and Biological Resource in Yarkant Oasis at Colleges and Universities Under the Department of Education of Xinjiang Uygur Autonomous Region;
关键词：
Punica granatum L;;preserved methods;;DNA extraction
摘  要：
Taking leaves of Punica granatum L.as materials,six methods of preserving fresh leaves were designed,genomic DNA was extracted by improved CTAB method,the effect of different preserving methods on genomic DNA extraction was studied.The results showed that the DNA extracted from leaves stored about 6months at the-70℃and5days at 4℃could get the same high quality comparing with the fresh leaves.Genomic DNA also could be extracted from sample stored 7and 14 days at-20℃,but the concentration was slightly lower than that extracted from fresh leaves.However,DNA from silica gel-dried sample was not obtained by the same way.After optimization of the DNA extraction method,the high-purity DNA from silica gel-dried sample was got,but the concentration of DNA were about 50%comparing with fresh leaves.Genomic DNA extracted from samples preserved as the above-mentioned six methods could meet the demands for SRAP amplification.Clear and perfect SRAP amplification patterns were obtained.

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