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当前位置: 首页 > 文章 > 鼠李糖乳杆菌发酵中草药复合枯草芽孢杆菌对黄羽肉鸡生长后期免疫性能及大肠杆菌感染的研究 中国畜牧兽医 2016,43 (6) 1523-1529
Position: Home > Articles > Research Lactobacillus rhamnosusFermented Herbal and Bacillus subtilis Complex on Immune Performance,E.coli Infection of Broiler China Animal Husbandry & Veterinary Medicine 2016,43 (6) 1523-1529

鼠李糖乳杆菌发酵中草药复合枯草芽孢杆菌对黄羽肉鸡生长后期免疫性能及大肠杆菌感染的研究

作  者：
谢全喜;张建梅;于佳民;亓秀晔;徐辉;徐海燕;谷巍
单  位：
山东宝来利来生物工程股份有限公司
关键词：
鼠李糖乳杆菌;发酵中草药;枯草芽孢杆菌;死亡率;免疫性能;盲肠菌群;大肠杆菌感染
摘  要：
试验旨在研究鼠李糖乳杆菌发酵中草药复合枯草芽孢杆菌对白羽肉鸡生长后期免疫性能及大肠杆菌感染的影响。选取体重相似、健康状况基本相同的360只1日龄肉鸡随机分成3个组,每组4个重复,每个重复30只。试验预试期7d,正试期35d。试验Ⅰ组为阳性对照组,试验Ⅱ组为阴性对照组,均饲喂基础日粮,试验Ⅲ组在基础饲料中添加1%发酵中草药制剂,且试验Ⅱ、Ⅲ组在肉鸡35日龄时腹腔注射大肠杆菌菌悬液1mL,连续攻毒2d,测定肉鸡死亡率、免疫器官指数、盲肠菌群含量、免疫球蛋白水平和白介素含量。结果表明,腹腔注射大肠杆菌造成鸡大量死亡,试验Ⅱ组高达75.00%,显著高于试验Ⅰ、Ⅲ组(P<0.05),使用发酵中草药微生态制剂后鸡死亡率显著下降(P<0.05),仅有23.33%;注射大肠杆菌后试验Ⅲ组脾脏指数和法氏囊指数显著升高(P<0.05),但使用发酵中草药微生态制剂后脾脏指数接近于试验Ⅰ组,而法氏囊指数稍高于试验Ⅰ组,说明发酵中草药微生态制剂可以抵抗大肠杆菌感染,脾脏和法氏囊发育不受影响;注射大肠杆菌后试验Ⅲ组大肠杆菌数量显著下降(P<0.05),但试验Ⅲ组由于饲喂发酵中草药复合芽孢杆菌制剂,肠道乳杆菌数量显著增加,抑制大肠杆菌的繁殖,从而大肠杆菌数量与正常饲喂状态下的试验Ⅰ组趋于一致;42日龄时,盲肠内容物sIgA含量试验Ⅲ组分别高出试验Ⅰ、Ⅱ组11.99%和36.56%(P<0.05),血清IgG含量试验Ⅲ组分别高出试验Ⅰ、Ⅱ组14.68%和28.15%(P<0.05);42日龄时,试验Ⅱ组IL-2含量最低,显著低于试验Ⅲ组(P<0.05),试验Ⅲ组IL-6含量显著低于试验Ⅱ组(P<0.05)。
译  名：
Research Lactobacillus rhamnosusFermented Herbal and Bacillus subtilis Complex on Immune Performance,E.coli Infection of Broiler
作  者：
XIE Quan-xi;ZHANG Jian-mei;YU Jia-min;QI Xiu-ye;XU Hui;XU Hai-yan;GU Wei;Shandong Baolai-Leelai Bioengineering Co.,Ltd.;
关键词：
Lactobacillus rhamnosus;;fermented herbs;;Bacillus subtilis;;mortality rate;;immune performance;;cecalmicrobiota;;E;;coli infection
摘  要：
This experiment was designed to study the complex of Lactobacillus rhamnosus fermented herbal and Bacillus subtilis on White Feather broiler immunity performance and impact of Escherichia coli infection.360one-day-old broiler chickens were randomly divided into 3groups with 4replicates in each group and 30 chickens per replicate.The pretrial period lasted for 7d,and the experiment lasted for 35 d.The chickens in the groupⅠ(positive control group)and groupⅡ(negative control group)were all only fed a basal diet,group Ⅲ was test group,by additive 1%fermented herbal preparations,groupsⅡ and Ⅲ were intraperitoneal injection of 1mL E.coli at35 d,broiler mortality,immune organ index,cecalmicroflora content,immunoglobulin levels,IL-2and IL-6content were tested.The results showed that injection of E.coli caused massive death of chickens,group Ⅱtook up to 75.00%,it was significantly higher than groups Ⅰ and Ⅲ(P<0.05),the mortality in group Ⅲ was significantly lower than group Ⅱ(P<0.05),was only23.33%.Injection of E.coli maked spleen index and thymus index of group Ⅱincreased significantly(P<0.05),the spleen index and thymus index of groups Ⅰ and Ⅲ were no significant difference(P>0.05).E.coli counts was significantly decreased after injectionin group Ⅲ(P<0.05),but the number of intestinal Lactobacilli of group Ⅲ was significantly increased(P<0.05),and inhibited the propagation of E.coli,the counts of E.coli in groupsⅠ and Ⅲ were no significant difference(P>0.05).At 42 d,the sIgA of the intestinal fluid in groupⅢ were higher than that of groupsⅠ and Ⅱ with 11.99% and 36.56%,respectively(P<0.05).The serum IgG concentrations of groupⅢ was higher than that of groupsⅠandⅡwith 14.68% and 28.15%,respectively(P<0.05).At 42 d,the IL-2content of group Ⅱ was the lowest,it was significantly lower than group Ⅲ(P<0.05),the IL-6of group Ⅲ was significantly lower than group Ⅱ(P<0.05).

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