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当前位置: 首页 > 文章 > Asymbiotic germination and low-temperature in vitro conservation of Cymbidium dayanum. 农业科学与技术(英文) 2008,9 (1) 67-69,74
Position: Home > Articles > Asymbiotic germination and low-temperature in vitro conservation of Cymbidium dayanum. Agricultural Science & Technology 2008,9 (1) 67-69,74

Asymbiotic germination and low-temperature in vitro conservation of Cymbidium dayanum.

作  者：
Luo YuanHua;Leng QingYun;Mo Rao;Chen YeYuan
单  位：
College of Agronomy; South China University of Tropical Agriculture; China.; Danzhou 571737
关键词：
cold;culture media;germplasm;in vitro culture;in vitro regeneration;micropropagation;nature conservation;plant genetic resources;protocorms;rooting;seed germination;seedlings;seeds;survival;tissue culture;Cymbidium
摘  要：
A research was conducted to establish asymbiotic germination and low-temperature in vitro conservation technique for Cymbidium dayanum by using plant tissue culture technique to realize its rapid propagation and long-term conservation in vitro. Using mature seeds of C. dayanum as explants, different media were selected to establish the asymbiotic germination technique system. With protocorms as materials, conservation, resumptive proliferation and plant regeneration conditions were selected to establish low-temperature in vitro conservation technique system preliminarily. The results showed that mature seeds of C. dayanum could germinate after culturing for 90 days on MS medium as well as Hyponex 1 medium. The germination rate reached more than 98%. Protocorms inoculated in Hyponex 1 medium could be conserved continuously at 5 degrees C in the dark for more than 18 months, and the survival rate could reach 90%. Conserved protocorms could realize resumptive proliferation culture both on 1/2-strength MS and Hyponex 1 media. The seedling-strengthening and rooting media were 1/2-strength MS media. This research provided a practical basis for in vitro conservation and rapid propagation of C. dayanum germplasm resource.

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