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Position: Home > Articles > Genetic Polymorphism of Wx Gene and Its Correlation with Main Grain Quality Characteristics in Rice Rice Science 2007,14 (2) 85-93

Genetic Polymorphism of Wx Gene and Its Correlation with Main Grain Quality Characteristics in Rice

作者：

Yat-wah Wan;Qi Deng;Shiquan Wang;Mingwei Liu;Hongyu Zhou;Ping L

单位：

Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China;Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, Chin

关键词：

waxy gene;simple sequence repeat;cleaved amplified polymorphic sequence;single nucleotide polymorphism;gelatinization temperature;gel consistency;amylose content;ric

摘要：

The allelic variation of the Wx gene in 50 non-glutinous rice varieties (lines) was analyzed by using the microsatellite marker RM190 [for (CT)_n simple sequence repeat (SSR)] and cleaved amplified polymorphic sequence(CAPS) marker 484/W2R-ACCI [for G/T single nucleotide polymorphism (SNP)]. Six homozygous (CT)_n types, namely (CT)_(20), (CT)_(19), (CT)_(18), (CT)_(17), (CT)_(16), (CT)_(14), (CT)_(11) and (CT)_(10), and a heterozygous genotype (CT)_(11)/(CT)_(18) were detected for RM190, of which (CT)_(11) and (CT)_(18) were predominant. Two homozygous Wx genotypes (G/G and T/T) and one heterozygous (G/T) were detected using 484/W2R-ACCI . Most of the materials with a RM190 of (CT)_(11) were G/G for SNP of 484/W2R-ACCI, while T/T for SNP was predominantly appeared in materials with (CT)_(18). The materials tested could be grouped into 10 categories using the two markers together. Results indicated that 59.3% variance of amylose content was attributed to the polymorphism of Wx gene revealed by RM190, while 56.1% and 24.6% of the variances in amylose content and gel consistency were respectively to the polymorphism of Wx gene revealed by 484/W2R-ACCI. Furthermore, with both SSR and CAPS markers, 72.4% of the variance in amylose content could be explained. In addition, the application prospects of the two markers in breeding were also discussed.

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Genetic Polymorphism of Wx Gene and Its Correlation with Main Grain Quality Characteristics in Rice

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