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当前位置: 首页 > 文章 > 绵羊IGFBP-6基因的克隆及序列分析 河南农业科学 2015,44 (4) 144-148
Position: Home > Articles > Cloning and Sequence Analysis of IGFBP-6 Gene in Sheep Journal of Henan Agricultural Sciences 2015,44 (4) 144-148

绵羊IGFBP-6基因的克隆及序列分析

作  者：
周明亮;杨平贵;吴登俊;张翔宇
单  位：
四川农业大学;四川省草原科学研究院;四川省畜牧科学研究院
关键词：
绵羊;IGFBP-6基因;克隆;生物信息学
摘  要：
以凉山半细毛羊为研究对象,克隆了其胰岛素样生长因子结合蛋白6基因(IGFBP-6),采用生物信息学方法进行遗传进化分析,对IGFBP-6蛋白的理化性质进行分析,并对其二级和三级结构进行预测。结果表明,绵羊IGFBP-6基因的CDS全长序列为711 bp,编码236个氨基酸,与牛、人、鼠的CDS同源性分别为96%、84%、74%,氨基酸序列同源性分别为95%、80%、69%;IGFBP-6蛋白大小为24.9 ku,理论等电点(p I)为8.83。遗传进化分析结果显示,绵羊IGFBP-6基因与山羊、牛等哺乳动物关系较近,与鱼类的亲缘关系较远。IGFBP-6蛋白存在明显的疏水性区域和亲水性区域,有1个信号肽、14个磷酸化位点、3个N-糖基化位点和7个O-糖基化位点;二级结构分析结果显示,IGFBP-6蛋白无规则卷曲、α-螺旋和β-折叠区域比例分别为77.54%、19.92%、2.54%;三级结构分析结果显示,IGFBP-6蛋白存在IGFBP_N和甲状腺球蛋白-Ⅰ型功能域。
译  名：
Cloning and Sequence Analysis of IGFBP-6 Gene in Sheep
作  者：
ZHOU Mingliang;YANG Pinggui;WU Dengjun;ZHANG Xiangyu;Sichuan Academy of Grassland Science;College of Animal Sci-Technology,Sichuan Agricultural University;Sichuan Animal Science Academy;
关键词：
sheep;;IGFBP-6 gene;;clone;;bioinformatics
摘  要：
The IGFBP-6 gene of Liangshan semi-fine wool sheep was cloned and anlayzed by bioinformatics method. The physicochemical characteristics of IGFBP-6 protein was anlayzed. The secondary structure and tertiary structure of IGFBP-6 protein were predicted. The results showed that the CDS sequence of IGFBP-6gene was 711 bp,encoding 236 amino acids. CDS homology was 96%,84% and 74% respectively,amino acid homology was 95%,80% and 69% respectively compared with bovine,human and rat. The amino acidsequence analysis revealed that its relationship was near with mammals,goat,cattle,and far with fish.The molecular weight was 24. 9 ku,isoelectric point was 8. 83,with obvious hydrophobic and hydrophilic regions,a signal peptide,one transmembrane region,forteen sites of phosphorylation,three sites of Nglycosylation and seven sites of O-glycosylation. There were forecast that the random coil,α-helix and β-sheet region were 77. 54%,19. 92%,2. 54% respectively in secondary structure,a IGFBP_N domain and a thyroglobulin-Ⅰtype domain were in tertiary structure.

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