Position: Home > Articles > A comparative study of three diagnostic methods for detection of canine parvovirus infection in dogs
Animal Husbandry & Veterinary Medicine
2008,40
(6)
31-35
3种犬细小病毒感染诊断方法比较
作 者：
宋永奇;李玉燕;吕艳丽;韩博;李伊立;贾君影;付丽娟
单 位：
中国农业大学动物医学院
关键词：
犬细小病毒抗原快速检测试剂盒;PCR;血凝/血凝抑制(HA/HI)试验;犬细小病毒(CPV)
摘 要：
本研究应用犬细小病毒抗原快速检测试剂盒、血凝/血凝抑制试验和PCR方法对120份具有腹泻/呕吐症状的患犬粪便样本进行了犬细小病毒检测。结果显示,3种方法检测阳性份数分别为60,49,68。犬细小病毒抗原快速检测试剂盒与血凝/血凝抑制(HA/HI)试验相比较,敏感性、特异性及总符合率分别为100.0%,84.5%,90.8%;与PCR法相比较,敏感性、特异性及总符合率分别为85.3%,96.2%,90.0%。从试验结果看,HA/HI法具有较高的特异性,但敏感性较低;PCR具有高度敏感性和特异性,但不适合临床推广;细小病毒抗原快速检测试剂盒,在正确操作下结果可靠,是临床诊断犬细小病毒感染的首选方法。
译 名：
A comparative study of three diagnostic methods for detection of canine parvovirus infection in dogs
作 者：
SONG Yong-qi,LI Yu-yan,LU Yan-li,HAN Bo,LI Yi-li,JIA Jun-ying,FU Li-juan(College of veterinary medicine,China Agricultral University,Beijing 100094,China)
关键词：
Antigen Rapid CPV Ag Test Kit;PCR;hemagglutination/hemagglutination inhibition(HA/HI);canine parvovirus(CPV)
摘 要：
Three diagnostic methods for canine parvovirus(CPV) infection were employed to test 120 feces samples collected from dogs with diarrhea and/or vomit in this study.The CPV positive samples were 60,49 and 68,respectively by detecting CPV antigen or nucleic acid using Antigen Rapid CPV Ag Test Kit,haemagglutination/haemagglutination inhibition(HA/HI) and PCR assay.The results of these three methods were evaluated.The percentages showed 100.0%,84.5% and 90.8%,respectively in relative sensitivity,specificity and overall agreement between Antigen Rapid CPV Ag Test Kit and HA/HI,and 85.3%,96.2% and 90.0 %,respectively between Antigen Rapid CPV Ag Test Kit and PCR.These results indicated that HA/HI was more specific than Antigen Rapid CPV Ag Test Kit but had a relatively lower sensitivity.PCR was sensitive and specific,but it was not a suitable test in clinic.The Antigen Rapid CPV Ag Test Kit was the first choice for detecting CPV infection if correctly used.
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