Position: Home > Articles > Establishment of TaqMan Probe Fluorescent RT-PCR Assay for Differential Detection of PRRSV Variant Strains
China Animal Health Inspection
2013
(1)
57-60
猪蓝耳病病毒变异株TaqMan探针实时荧光定量RT-PCR鉴别诊断方法的建立
作 者：
晏勇邦;龚中贵;王俊峰;邓理主;王林川
单 位：
华南农业大学;韶关市动物卫生监督所
关键词：
猪蓝耳病病毒变异株;荧光RT-PCR;TaqMan探针
摘 要：
根据GenBank收录的PRRSV基因序列,设计一对引物F、R和一条TaqMan荧光探针P,建立了猪蓝耳病病毒荧光RT-PCR检测方法。该方法敏感性比常规的RT-PCR高100倍,对PRRSV细胞培养物的检测下限可以达到2个TCID50,能特异检测出蓝耳病病毒,并且可以鉴别诊断PRRSV变异株和经典株,同时根据灵敏度试验建立的标准曲线,能够对PRRSV RNA进行相对定量。
译 名：
Establishment of TaqMan Probe Fluorescent RT-PCR Assay for Differential Detection of PRRSV Variant Strains
作 者：
Yan Yongbang 1 , Gong Zhonggui 1 , Wang Junfeng 1 , Deng Lizhu 1 , Wang Linchuan 2 (1.Shaoguan CityAnimal Health Supervision Institute,Shaoquan,Guangdong 512026; 2.South China Agricultural University,Guangzhou,Guangdong 510642)
关键词：
PRRSV;RT-PCR;TaqMan
摘 要：
A method to detect porcine reproductive and respiratory syndrome virus (PRRSV) was developed using fluorescent RTPCR method. Based on the published PRRSV genomic sequence in GenBank,a pair of primers F,R and a TaqMan fluorescent probe P were designed. This method was more sensitive than conventional RT-PCR by 100-fold. It can detect cell culture material of PRRSV as little as 2 TCID 50 . With high specificity,it could well differentiate variant and classical PRRSV strains. Besides,it could relatively quantificate PRRSV RNA according to the established standard curve of sensitivity experiment.
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