Position: Home > Articles > Study on the purity detection of mulberry silkworm ( Bombyx mori ) varieties by applying the RAPD molecular marking technique
Hubei Agricultural Sciences
2002
(3)
56-58
RAPD分子标记技术应用于家蚕品种纯度检测的研究
作 者：
廖富蘋;林健荣;莫红丽;余爱群;文哲光
单 位：
广东省丝绸集团公司;广东省丝绸集团公司 广州510642;华南农业大学蚕业服装系
关键词：
家蚕蚕种;杂种纯度;RAPD技术;检测
摘 要：
对家蚕品种“932”、“75 32”、芙蓉、湘晖等的原种 ,“932”×芙蓉、“75 32”×湘晖的杂交原种 ,(“932”×芙蓉 )× (“75 32”×湘晖 )F1杂交种进行RAPD分子标记纯度检测筛选 ,经过 180个随机引物的筛选 ,获得可用于判别“932”×芙蓉、“75 32”×湘晖杂交原种、(“932”×芙蓉 )× (“75 32”×湘晖 )四元杂交种纯度的分子标记 ,初步建立了从DNA提取到纯度判别的技术方法。
译 名：
Study on the purity detection of mulberry silkworm ( Bombyx mori ) varieties by applying the RAPD molecular marking technique
作 者：
Liao Fupin et al. (Department of Sericulture and Fashion Design,South China Agricultural University,Guangzhou 510642,China)
关键词：
mulberry silkworm egg,hybrid purity,RAPD technique,detection
摘 要：
By applying RAPD molecular marking technique and through the selection of 180 random primers,a technical method was preliminary established to extract the DNA and to detect the purity of mulberry silkworm ( Bombyx mori ) eggs of parental varieties named 932,7532,Furong and Xianghui,parental hybrids named 932×Furong and 7532×Xianghui,and the quaternary hybrid named (932×Furong)×(7532×Xianghui).
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