当前位置: 首页 > 文章 > HPLC法测定芦荟体内芦荟苷、芦荟大黄素含量的研究 河北农业大学学报 2005,28 (2) 61-64
Position: Home > Articles > On determination of Aloin and Aloe-emodin in Aloe barbadensis mill by HPLC Journal of Agricultural University of Hebei 2005,28 (2) 61-64

HPLC法测定芦荟体内芦荟苷、芦荟大黄素含量的研究

作  者:
马晶军;方正;吴秋华;孙奂明;温雅君
单  位:
河北农业大学;北京农业技术推广站
关键词:
HPLC;芦荟苷;芦荟大黄素
摘  要:
通过对比试验探讨了对芦荟中芦荟苷、芦荟大黄素的提取方法。对HPLC法同时测定芦荟体内芦荟苷、芦荟大黄素含量的方法进行了探讨。分别对流动相、检测波长等条件进行了优化,并进行了仪器精密度试验、方法精密度试验以及回收率测定,最终确立了应用HPLC测定芦荟体内芦荟苷、芦荟大黄素含量的色谱条件。
译  名:
On determination of Aloin and Aloe-emodin in Aloe barbadensis mill by HPLC
作  者:
MA Jing-jun~1,FANG Zheng~1,WU Qiu-hua~1,SUN Huan-ming~2,WEN Ya-jun~2(1.Agricultural University of Hebei,Baoding 071001, China;2.Beijing Agro-Tech Extension Station, Beijing 100029, China)
关键词:
HPLC; aloin; aloe-emodin
摘  要:
The extraction methods of aloin and aloe-emodin in Aloe barbadensis was explored by contrast experimentation. A method for the determination of aloin and aloe-emodin in Aloe barbadensis by HPLC was developed. After the optimization of many conditions, such as mobile phase, detection wavelengh and so on, and the precision experimentations of instrument and method, as well as the determination of recovery rate, the best chromatography conditions was established.

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