COMPARISON ON Cry1Ab PROTEIN CONTENT IN TRANSGENIC SORGHUM

ZHANG Ming-zhou~1 CHEN Zong-lun~1 FANG Mei-ming~1 CUI Hai-rui~2 XIA Ying-wu~2 (1.College of Life Science,China Jiliang University,Hangzhou,Zhejiang 310018;2.Institute of Nuclear Agricultural Sciences,Zhejiang University,Hangzhou,Zhejiang 310029)

transgenic sorghum;Cry1Ab protein;cry1Ab gene;Agrobacterium tumefaciens

The insecticidal Bacillus thuringiensis(Bt) cry1Ab gene was introduced into sorghum restoring line 115 efficiently mediated by Agrobacterium tumefaciens.A total of 13 independent transgenic plant lines and 26 transgenic plants were regenerated with the average transformation efficiency of 5.1%.The results of GUS expression,PCR analysis,Southern and Western hybridization showed that the cry1Ab gene had been integrated and expressed exactly in sorghum.The expression level of cry1Ab gene was detected in transgenic sorghum plants by using ELISA kits.There were obvious differences in Cry1Ab protein content with the different transgenic plants,and the highest content was 0.850μg/g,which accounted for 0.016% of the total soluble protein,while no Cry1Ab protein was detected in some transgenic plants.There were also obvious differences in Cry1Ab protein content in the different organs of the same transgenic plants,following the order of leaf>glumes>seed>root>stem.The Cry1Ab protein content in middle leaf was higher than that in base and top leaf.