Mycorrhizal morphology of fungus-infected wild Vaccinium bracteatum Thunb. in Hunan Province

ZHU Jianghua;WANG Sen;CHANG Shuangshuang;LI Chang;Lü Fangde;Jiangxi Environmental Engineering Vocational College;Central South University of Forestry and Technology;Hainan New Green God Tropical Biological Engineering Co.,Ltd.;

wild Vaccinium bracteatum Thunb.;;ericoid mycorrhiza(ERM);;mycorrhizal fungi;;trypan blue staining;;Hunan Province

Field samplings of mycorrhiaz from wild Vaccinium bracteatum plants were conducted in Hunan Province and the infection status of mycorrhizal fungi was studied by the trypan blue staining-squash method.The results showed the following:(1) The wild plants of V.bracteatum in Hunan Province were infected by mycorrhizal fungi.The infection rate varied from 38.60% to 63.80% with a maximum difference in the infection rate of 25.20%;(2) When the soil pH value was between 4 and 6,the infection rate of mycorrhizal infection rate increased with the increase of pH value;the highest mycorrhizal infection rate(63.80%) was observed in Huangjiapo,Yueyang City where the soil pH value was 5.82.When the soil pH value was greater than 6,the mycorrhizal infection rate decreased with the increase of pH value;(3) Multiple ectomycorrhizal invagination points were observed on the same segment,and the fungi invaded across cells,while endogenous hyphae inverted appeared as a "Y"-type structure throughout the whole root segment;and(4) The mycelium-infected cells formed a"hyphal connecting bridge"and the epidermal cells were lost as a result of the mycorrhizal fungi infection.