当前位置: 首页 > 文章 > 红哺鸡竹丛生芽诱导的初步研究 湖南林业科技 2009,36 (1) 18-20
Position: Home > Articles > Study on clustered bud induction of Phyllostachys iridenscens Hunan Forestry Science & Technology 2009,36 (1) 18-20

红哺鸡竹丛生芽诱导的初步研究

作  者:
宋晓娣;陈建华
单  位:
中南林业科技大学
关键词:
红哺鸡竹;丛生芽;褐变控制
摘  要:
以红哺鸡竹为材料,以MS为基本培养基,通过对外植体不同的消毒方法和防褐变处理,附加不同种类和浓度的植物生长调节物质诱导丛生芽。结果表明:红哺鸡竹带芽节段最佳消毒方式是75%酒精浸泡30 s,用0.1%升汞处理6 m in。红哺鸡竹丛生芽诱导的最佳培养基是:MS+6-BA(5 mg/L)+TDZ(0.1 mg/L)。在培养基中加入PVP(1 g/L)有明显的防褐变效果,外植体在半胱氨酸(100 mg/L)溶液浸泡30 m in以及暗培养能有效降低褐化率。
译  名:
Study on clustered bud induction of Phyllostachys iridenscens
作  者:
SONG Xiaodi,CHEN Jianhua (Central South University of Forestry and Technology,Changsha 410004,China)
关键词:
Phyllostachys iridenscens;clustered buds;browning prevention
摘  要:
Using Phyllostachys iridenscens as the research material and MS as the basic medium.Through the different methods of disinfection,anti-browning treatment and added different types of concentration of plant growth regulators to induce buds.The result showed that the best disinfectant is to soak for 30 s with 75% alcohol and to deal with for 6 min with 0.1% mercuric chloride.The best medium is MS+6-BA(5 mg /L)+TDZ(0.1 mg /L).Adding PVP into the medium can bring obvious anti-browning effect.Soaking explant for 30 min in 100 mg /L cysteine and dark treatment can effectively ligten browning rate.

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