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当前位置: 首页 > 文章 > 达氟沙星对史氏鲟红细胞抗氧化功能及微核形成的影响 水产学报 2006,30 (5) 17-22
Position: Home > Articles > Effect of danofloxacin on antioxidant function and micronucleus formation of erythrocyte in Acipenser schrencki Journal of Fisheries of China 2006,30 (5) 17-22

达氟沙星对史氏鲟红细胞抗氧化功能及微核形成的影响

作  者：
卢彤岩;徐连伟;刘红柏;赵吉伟;杨雨辉;佟恒敏
单  位：
中国水产科学研究院黑龙江水产研究所;海南大学生命科学与农学院;东北农业大学
关键词：
史氏鲟;红细胞;抗氧化;微核;达氟沙星
摘  要：
2004年5月至7月间,用20mg.kg-1、50mg.kg-1和100mg.kg-1剂量对史氏鲟经口灌服达氟沙星20d,分别于第5、10、15和20天定红细胞SOD、CAT、Na+、K+-ATP酶、GST、GSH-PX等抗氧化酶活性及丙二醛(MDA)含量,以评价达氟沙星对史氏鲟抗氧化防御功能的影响,同时进行红细胞微核率和总核异常率的测定。结果表明实验第5天各实验组红细胞SOD活性均显著高于对照组;各实验组红细胞中CAT的活性明显高于对照组;红细胞Na+、K+-ATP酶活性没有明显的改变。实验第20天时100mg.kg-1剂量组GSH-PX活性明显高于对照组及其他剂量组(P<0.05);GST活性及MDA含量没有明显的影响。达氟沙星在实验剂量内对史氏鲟红细胞核微核率没有明显的影响,但核异常率呈明显升高。
译  名：
Effect of danofloxacin on antioxidant function and micronucleus formation of erythrocyte in Acipenser schrencki
作  者：
LU Tong-yan~1,XU Lian-wei~2,LIU Hong-bai~1,ZHAO Ji-wei~1,YANG Yu-hui~3,TONG Heng-min~2(1.Heilongjiang River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Harbin 150070,China;2.Northeast Agriculture University,Harbin 150030,China;3.Life Science and Agricultural College,Hainan University,Haikou 570228,China)
关键词：
Acipenser schrenscki Brand;erythrocyte;antioxidation;micronucleus;danofloxacin
摘  要：
The effects of danofloxacin by oral administration for 20 days at dosage of 20,50 and 100 mg·kg~(-1) on the toxicity of Acipenser schrencki[WTB1] were studied.The activity of superoxide dismutase(SOD),catalase(CAT),Na~+,K~+-ATPase,glutathione-S-transferase(GSH-PX),glutathione peroxidase(GST) and Maleic Dialdehyde(MDA) content were investigated by kit,so as to appraise security of danofloxacin used in Acipenser schrencki.Meanwhile blood smears were prepared to investigated the micronucleus and nuclear anomalies rate.The results showed:the SOD activity in erythrocyte remains higher level than that of control group;CAT activity in erythrocyte is higher than control group obviously;The activity of Na~+, K~+-ATPase in erythrocyte is not significant compared with control group;The GSH-PX activity in erythrocyte of 100 mg·kg~(-1) is higher than other test groups(P<0.05);the MDA concentration in erythrocyte remains unchanged;the activity of GST in erythrocyte is not affected by danofloxacin;The erythrocyte micronucleus remained stable,but nuclear anomalies in test group rose markedly compared with control group.The result showed that the nuclear anomalies are the highest at 10th day,then began to decrease but still is higher obviously than control group.The result showed that danlfloxacin affected SOD,CAT,GSH-PX activity,does not changed the Na~+,K~+-ATPase,GST activity and MDA content.The nuclear anomalies rate of test fish is higher than control group.

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