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Position: Home > Articles > Establishment of Tissue culture and Rapid Propagation System in Ficus elastica Journal of Anhui Agricultural Sciences 2008,36 (1) 81-82

橡皮树组织培养快繁体系的建立

作  者:
赵海清;王晓军
单  位:
中国科学院新疆理化技术研究所
关键词:
橡皮树;茎尖外植体;组织培养;快速繁殖
摘  要:
[目的]利用组培快繁技术培育橡皮树,以满足花卉市场对该植物的需求。[方法]以橡皮树茎尖为外植体,选择不同培养基和激素配比,初步建立橡皮树组培快繁体系,并进行对比试验。[结果]结果表明,愈伤诱导和芽分化培养基为:MS+6-BA 1.0 mg/L+NAA0.01 mg/L,丛生芽增殖培养基为:MS+6-BA 2.0 mg/L+NAA 0.01 mg/L,最佳生根培养基为1/2 MS+0.01 mg/L NAA+0.5 mg/L IBA。[结论]该研究为橡皮树的规模化生产提供了实践基础,但橡皮树的体细胞胚的诱导及萌发技术仍需改进。
译  名:
Establishment of Tissue culture and Rapid Propagation System in Ficus elastica
作  者:
ZHAO Hai-qing et al (Xinjiang Technical Institute of Physics and Chemistry,Chinese Academy of Science,Urumqi,Xinjiang 830011)
关键词:
Ficus elastica;Shoot tip explants;Tissue culture;Rapid propagation
摘  要:
[Objective] The aim of the research was to breed Ficus elastica by using tissue culture and rapid propagation technologies and meet the demands of flower market to this plant.[Method] With shoot tip of Ficus elastica as explants,different medium and hormone combinations were selected to set up the tissue culture and rapid propagation system of F.elastica preliminarily and make a comparative test.[Result] The medium for callus induction and bud differentiation was MS+1.0 mg/L 6-BA+0.01 mg/L NAA,the medium for the propagation of clumpy buds was MS+2.0 mg/L 6-BA+0.01 mg/L NAA and the optimum rooting medium was 1/2 MS+0.01 mg/L NAA+0.5 mg/L IBA.[Conclusion] This research provided practical basis for mass production of F.elastica.But the induction and germination technologies of somatic embryo still need to be improved.

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