作 者：
刘文婷;王伟;易华西;章检明;韩雪;张兰威;何胜华
单 位：
哈尔滨工业大学化工与化学学院;中国海洋大学食品科学与工程学院
关键词：
乳酸菌细菌素;荧光假单胞菌;生物膜;抑制
摘 要：
细菌素Lac-B23是由植物乳杆菌Lactobacillus plantarum B23产生的具有广谱抑菌活性的小分子肽,对嗜冷腐败菌荧光假单胞菌具有明显的抑制作用。细菌素Lac-B23对荧光假单胞菌的抑菌作用研究结果表明,细菌素Lac-B23可以使荧光假单胞菌胞内无机磷和三磷酸腺苷(adenosine triphosphate,ATP)发生不同程度的泄漏;细菌素Lac-B23首先破坏荧光假单胞菌的细胞外膜,然后消散了荧光假单胞菌细胞内膜跨膜电位(Δφ)和pH值梯度(DpH),细胞外膜渗透性增强,使细胞膜形成孔道。扫描电子显微镜和透射电子显微镜结果表明荧光假单胞菌细胞形态完整性发生改变,细胞膜破坏,从而导致细胞死亡。细菌素Lac-B23能够破坏荧光假单胞菌生物膜中的网络结构,对生物膜具有明显的破坏作用。
译 名：
Anti-Biofilm and Antimicrobial Activity of Bacteriocin Lac-B23 from Lactic Acid Bacteria against Pseudomonas fluorescens
作 者：
LIU Wenting;WANG Wei;YI Huaxi;ZHANG Jianming;HAN Xue;ZHANG Lanwei;HE Shenghua;College of Chemical Engineering and Chemistry,Harbin Institute of Technology;School of Food Science and Engineering,Ocean University of China;
单 位：
College of Chemical Engineering and Chemistry,Harbin Institute of Technology%School of Food Science and Engineering,Ocean University of China
关键词：
lactic acid bacteria;;bacteriocin;;Pseudomonas fluorescens;;biofilm;;inhibition
摘 要：
The aim of this study was to elucidate the mode of action of a novel bacteriocin Lac-B23 fromLactobacillus plantarum B23,a small peptide with broad-spectrum antimicrobial activity,on Pseudomonas fluorescens.P.fluorescens was susceptible to bacteriocin Lac-B23 action,leading to the release of intracellular ATP and inorganic phosphate.Bacteriocin Lac-B23 first destroyed the extracellular membrane and then dissipated both transmembrane potential(Δφ) and transmembrane pH gradient(DpH),leading to the formation of channels in the cell membrane.Clear changes in the morphological integrity of P.fluorescens were observed under scanning electron microscopy(SEM) and transmission electron microscopy(TEM).The cell membrane was destroyed,leading to the death of cells.Bacteriocin Lac-B23 could affect the network structure of the biofilm produced by P.fluorescens and exhibited anti-biofilm activity.
