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当前位置: 首页 > 文章 > 实验感染血吸虫牛、兔抗体的消长规律 浙江农业学报 2009,21 (4) 316-320
Position: Home > Articles > Anti-schistosome antibody levels in blood serum of experimentally infected cattle and rabbits before and after treatment Acta Agriculturae Zhejiangensis 2009,21 (4) 316-320

实验感染血吸虫牛、兔抗体的消长规律

作  者：
卢福庄;付嫒;赵俊龙;顾小根;俞国乔;张雪娟
单  位：
浙江省农业科学院;浙江省畜牧兽医局;华中农业大学
关键词：
血吸虫;黄牛;兔;抗体水平;金标免疫渗滤法试剂盒
摘  要：
用家畜血吸虫病金标免疫渗滤法试剂盒(以下简称试剂盒)检测实验感染血吸虫治疗前后3头牛和3只兔的血清中的血吸虫抗体水平。结果表明,1号、2号和3号牛分别于感染后第3周、第4周和第2周检测到血吸虫抗体,于治疗后第29周、第27周和第39周抗体转阴。1号、2号和3号兔分别于感染后第3周、第3周和第4周检测到抗体,于治疗后第35周、第17周和第39周抗体转阴。用试剂盒检测可比粪孵毛蚴法提早2周检出病畜。表明本试剂盒可用于家畜血吸虫病的早期诊断,并且在患血吸虫病家畜治疗10个月后,用本试剂盒的检查具有疗效考核价值。
译  名：
Anti-schistosome antibody levels in blood serum of experimentally infected cattle and rabbits before and after treatment
作  者：
LU Fu-zhuang1,FU Yuan1,ZHAO Jun-long2,GU Xiao-gen3,YU Guo-qiao3,ZHANG Xue-juan1(1Institute of Animal Husbandry and Veterinary Sciences,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China;2College of Animal Medicine,Huazhong Agricultural University,Wuhan 430070,China;3Zhejiang Provincial Bureau of Animal Husbandry & Veterinary Medicine,Hangzhou 310020, China)
关键词：
schistosome;cattle;rabbit;antibody level;dot immuno-gold filtration assay
摘  要：
Anti-schistosome antibody levels in blood serum of experimentally infected 3 cattle and 3 rabbits before and after treatment were detected by Reagent Kit of DIGFA for Animal Schistosomiasis(RKDAS).Anti-schistosome antibodies could be detected with RKDAS when Cattle 1,Cattle 2 and Cattle 3 had been infected by schistosome cercaria for 3,4 and 2 weeks,respectively.The antibodies could not be detected after the cattle had been treated with Praziquantel for 29,27 and 39 weeks,respectively.Meanwhile,the anti-schistosome antibodies could be detected by RKDAS when Rabbit 1,Rabbit 2 and Rabbit 3 had been infected by schistosome cercaria for 3,3 and 4 weeks,respectively.The antibodies could not be detected after the rabbits had been treated with Praziquantel for 35,17 and 39 weeks,respectively.Compared with the feces hatching miracidium method,RKDAScould detect the diseased cattle and rabbits which were infected by schistosome 2 weeks earlier.The results suggested that RKDAScould be used for early detection of schistosomiasis in livestock.If the livestock infected with schistosome had been treated with medicine for more than 10 months,the detection results of RKDAS had an assessable therapeutic effect.

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