作 者：
梁芳;黄秋伟;檀小辉;龙凌云;张继;邓旭
单 位：
广西壮族自治区亚热带作物研究所;玉林师范学院
关键词：
红花玉蕊;水体盐度;胁迫;生理特性;潮汐系统
摘 要：
【目的】分析潮汐系统下不同盐度水体胁迫对红花玉蕊幼苗生理特性的影响,为美化水岸环境及恢复红树林和湿地植被提供参考依据。【方法】模拟半日潮,每天在温室分别以不同盐度(5‰、10‰、15‰、20‰、25‰、30‰、35‰和40‰)水体对一年生红花玉蕊实生苗进行完全浸没胁迫处理12 h,以浸泡0盐度水体为对照(CK),处理后第3 d开始调查各处理幼苗的形态指标,处理后第7 d取样测定各处理的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性、丙二醛(MDA)和脯氨酸(Pro)含量及叶绿素荧光参数(Fv/Fm),综合分析红花玉蕊的耐盐性。【结果】胁迫红花玉蕊幼苗的水体盐度超过20‰时,其叶片脱落率迅速增加。5‰盐度水体协迫处理红花玉蕊幼苗叶片的SOD活性显著低于CK(P<0.05,下同),随着水体盐度的升高,SOD活性总体上呈升高趋势,但均低于CK。当水体盐度高于15‰时,POD活性均显著高于CK,并在水体盐度30‰时达最高值。MDA含量随水体盐度升高呈先升后降的变化趋势,水体盐度为25‰时MDA含量最高,在40‰时最低,但与CK相比,各盐度水体协迫处理的MDA含量均无显著差异;Pro含量随水体盐度的升高而升高,但水体盐度为5‰时,Pro含量比CK稍微上升,说明红花玉蕊可能也适合在5‰水体盐度下生长。Fv/Fm随水体盐度的升高而降低,与Pro含量和叶片脱落率的变化趋势相反。【结论】红花玉蕊幼苗能适应5‰盐度水体淹浸12 h胁迫;在不同盐度水体胁迫下的叶片脱落率和Fv/Fm均可作为耐受水体盐度胁迫伤害程度的参考指标;POD活性和Pro含量可作为红花玉蕊幼苗耐受盐度胁迫响应的生理指标。
译 名：
Effects of different water salinities on physiological characteristics of Barringtonia acutangula seedlings under tidal system
作 者：
LIANG Fang;HUANG Qiu-wei;TAN Xiao-hui;LONG Ling-yun;ZHANG Ji;DENG Xu;Yulin Normal University;Guangxi Subtropical Crops Research Institute;
关键词：
Barringtonia acutangula;;water salinity;;stress;;physiological characteristics;;tidal system
摘 要：
【Objective】The effects of different water salinities stresses on the physiological characteristics of Barringtonia acutangula seedlings under tidal system were analyzed,which provided reference for beautifying the waterfront environment and vegetation restoration of mangroves and wetlands.【Method】Simulated half-day tide,one-year-old B. acutangula seedlings in the greenhouse were treated by different salinities(5‰,10‰,15‰,20‰,25‰,30‰,35‰ and 40‰).The whole immersion stress treatment was carried out for 12 h,and the 0‰ salinity water was used as the control(CK).The morphological indexes of treated seedlings were investigated on the 3 rdday after treatment,and superoxide dismutase(SOD)activity,peroxidase(POD)activity,malondialdehyde(MDA)content,proline content and chlorophyll fluorescence parameters were sampled on the 7 thday after treatment and were comprehensively analyzed for salt tolerance of B.acutangula.【Result】When the water salinity exceeded 20‰ during the stress,the leaf drop rate increased rapidly. The SOD activity of the leaves treated with 5‰ salinity was significantly lower than that of CK(P<0.05,the same below).With the increase of salinity,the activity of SOD increased generally,but they were lower than CK. When the salinity of the water was higher than 15‰,the POD activity was significantly higher than that of CK,and reached the peak when thesalinity of the water was 30‰. The content of MDA increased firstly and then decreased with the increase of salinity. The MDA content was the highest when the salinity was 25‰,and the lowest at the 40‰. But there was no significant difference in the MDA content of each salinity compared with CK. The Pro content increased with the increase of salinity,but when the salinity of the water was 5‰,the Pro content increased slightly compared with CK,indicating that the B. acutangula might also be suitable for living in the salinity water of 5‰. Fv/Fmdecreased with increase of salinity,and was contrary to the change trend of Pro content and leaf drop rate.【Conclusion】The seedlings of B. acutangula have strong adaptability to the flooding of 5‰ salinity water for 12 h. Under different water salinities stresses,the leaf drop rate and Fv/Fm can be used as the indexes for damage degree by tolerance to salinity stress.The POD activity and Pro content can be used as physiological indicators for salinity stress response of B. acutangula.
