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当前位置: 首页 > 文章 > 湖北海棠二价融合表达载体MhT2AC的构建及转化烟草耐盐性分析 园艺学报 2012,39 (1) 1-12
Position: Home > Articles > Construction of a Divalent Plant Binary Expression Vector MhT2AC from Malus hupehensis and Analysis of Salinity Tolerance in the Tobacco Transformants Acta Horticulturae Sinica 2012,39 (1) 1-12

湖北海棠二价融合表达载体MhT2AC的构建及转化烟草耐盐性分析

作  者：
张计育;王三红;乔玉山;渠慎春;郭忠仁;章镇
单  位：
江苏省中国科学院植物研究所;南京农业大学园艺学院
关键词：
湖北海棠;二价融合表达载体;MhTGA2转录因子;几丁质酶基因;耐盐性
摘  要：
利用口蹄疫病毒2A序列将湖北海棠MhTGA2转录因子和几丁质酶基因(Mhchit1)两个抗病相关基因融合在同一个开放阅读框下,构建二价融合植物双元表达载体,命名为MhT2AC。将该载体转化烟草,获得了8个转基因株系,MhTGA2基因和Mhchit1基因在烟草中共表达,并且转基因株系中NtSOD、NtAPX和NtPPO等抗逆基因的表达量均加强;转基因烟草T1代植株抗盐性增强。
译  名：
Construction of a Divalent Plant Binary Expression Vector MhT2AC from Malus hupehensis and Analysis of Salinity Tolerance in the Tobacco Transformants
作  者：
ZHANG Ji-yu 1,2 ,WANG San-hong 1 ,QIAO Yu-shan 1 ,QU Shen-chun 1 ,GUO Zhong-ren 2,* ,and ZHANG Zhen 1,* (1College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China)
关键词：
Malus hupehensis;MhT2AC;MhTGA2;Mhchit1;salinity tolerance
摘  要：
The plant binary expression vector containing the divalent genes of MhTGA2 and Mhchit1 isolated from Malus hupehensis and fused in a single open reading frame(ORF)by Foot and mouth disease virus(FMDV)were constructed.The vector was then introduced into tobacco via Agrobacterium-mediated.The expression of NtSOD,NtAPX and NtPPO genes were detected and salinity tolerance was evaluated in the tobacco transformants.The results showed that we successfully constructed a plant binary expression vector with bivalent fusion and denoted it as MhT2AC.The MhTGA2 gene and the Mhchit1 gene were co-expression in the transformants.Comparing with the controls,the expression of NtSOD,NtAPX and NtPPO genes were enhanced in transgenic lines.Moreover,the salinity tolerance of the T1 transformants were significantly improved.

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