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Position: Home > Articles > Effect of Herba Cistanchis on the Expression of the BMP2 Gene in Rat Osteoblasts China Animal Husbandry & Veterinary Medicine 2013,40 (1) 17-21

肉苁蓉水提液对大鼠成骨细胞骨形态发生蛋白2基因表达的影响

作  者:
邢晓旭;刘钟杰;韩博
单  位:
太原动物园;中国农业大学动物医学院
关键词:
肉苁蓉;成骨细胞;BMP2基因;荧光定量PCR
摘  要:
本试验采用酶消化法分离得到大鼠颅骨成骨细胞,取第3代成骨细胞,通过加入不同浓度的肉苁蓉水提液(5×10-5~5×10-1 mg/mL)来提取细胞总RNA,再采用实时荧光定量PCR方法,来观察不同浓度的肉苁蓉水提液对大鼠成骨细胞骨形态发生蛋白2(bone morphogenetic protein 2,BMP2)基因表达的变化。从试验结果可以得出,肉苁蓉水提液对大鼠成骨细胞BMP2基因表达具有促进作用。
译  名:
Effect of Herba Cistanchis on the Expression of the BMP2 Gene in Rat Osteoblasts
作  者:
XING Xiao-xu1,LIU Zhong-jie2,HAN Bo2(1.Taiyuan Zoo,Taiyuan 030009,China; 2.College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)
关键词:
Herba Cistanchis;osteoblast;BMP2 gene;fluorescent quantitative PCR
摘  要:
The objection of the study was to investigate the effect of Herba Cistanchis aqueous extracts on the bone morphogenetic protein 2(BMP2) gene expression in rat osteoblasts.Osteoblasts were isolated from calvaria in neonatal rat and exposure to different doses of Herba Cistanchis aqueous extracts(5×10-5 to 5×10-1 mg/mL).RNA was isolated from osteoblasts cultured and BMP2 gene expression was detected by Real-time fluorescent quantitative PCR.The results indicated that Herba Cistanchis aqueous extracts stimulated BMP2 gene expression.

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