摘 要：
本文对繁枝蜈蚣藻中可溶性粗多糖的提取工艺进行了研究,通过单因素试验和L9(33)正交试验,研究了料液比、温度、时间对多糖提取率的影响,结果显示温度和料液比是影响多糖提取率的主要因素,最佳工艺为料液比1:60,温度100℃,时间3h,在最佳提取工艺时,繁枝蜈蚣藻的多糖提取率为22.527%。采用紫外检测和茚三酮法检测未见多糖含有核酸、蛋白质。
译 名：
Optimum Extraction Process of Polysaccharides from Grateloupia filicina by Orthogonal Test
作 者：
ZHU Liang,ZHANG Qing,WANG Yi-fei,CEN Ying-zhou (Biomedical Research and Development Center, Jinan University, Guangzhou 510632, China)
关键词：
Grateloupia filicina;soluble polysaccharide;extraction technology
摘 要：
This paper studied the extraction process of polysaccharide from Grateloupia filicina. Single factor test and orthogonal experiment design methods L9(33) were applied to analyze the effects of the factors such as solid-liquid ratio and temperature and time on the extraction percentage of the polysaccharides. Experimental results indicated that the temperature and solid-liquid ratio would significantly affect the extraction percentage of the soluble polysaccharides. The optimum solid-liquid ratio was: 1:60, the temperature 100℃ and the extraction time 3h. In the conditions, the extraction percentage of soluble polysaccharides in Grateloupia filicina was 22.527%. No nuclear-acid and protein has been found in the polysaccharides by ultraviolet detection and Ninhydrin reaction .
