Inhibitory Effect of Malvidin-3-Galactoside Chloride on TNF-α-Induced Inflammation in Endothelial Cells

WANG Jian;HUANG Wu-yang;LI Chun-yang;ZHENG Qi-sheng;LIU Ya-mei;Institute of Farm Product Processing,Jiangsu Academy of Agricultural Science;College of Food Science and Technology,Nanjing Agricultural University;National Technical Research Centre of Veterinary Biological Products,Jiangsu Academy of Agricultural Science;

malvidin-3-galactoside chloride(Mv-3-gal-Cl);;human umbilical vein endothelial cell(HUVEC);;tumor necrosis factor-α;;inflammatory effect;;intercellular cell adhesion molecule-1(ICAM-1);;NF-kappa B

Objective: The effect of malvidin-3-galactoside chloride(Mv-3-gal-Cl) on TNF-α-induced inflammation in human umbilical vein endothelial cells(HUVEC) was investigated in this study. Methods: HUVEC were treated with TNF-α(10 μg/L) alone and combined with Mv-3-gal-Cl(1, 10, 50 and 100 μmol/L), respectively. DMSO was used as control. The soluble ICAM-1 protein content in cell supernatant wa s detected by ELISA. The change in ICAM-1 mRNA expression level in cells was detected by RT-PCR. The expression of ICAM-1 and IкBα was assessed by Western blotting. The activity of NF-кB was evaluated by immunofluorescence. Results: Mv-3-gal-Cl appeared to specifically down-regulate the TNF-α-induced cell surface expression of ICAM-1 and ICAM-1 mRNA expression and protein release from HUVEC cells in a concentration-dependent manner. The inhibitory effect of Mv-3-gal-Cl on protein expression might be associated with the suppression of NF-κB in HUVECs. Conclusion: Mv-3-gal-Cl can inhibit TNF-α-induced inflammation in HUVECs. The anti-inflammatory effect of Mv-3-gal-Cl provides a theoretical basis for preventing and treating pro-inflammatory cytokinesinduced endothelial dysfunction and cardiovascular diseases resulting from inflammation.