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Position: Home > Articles > Research on Tissue culture and the Holographic Phenomenon of Lactuca sativa var.capitata L. Northern Horticulture 2007 (1) 142-144

生菜子叶组织培养及全息现象的研究

作  者:
宋英今;季静;刘海学;王罡
单  位:
天津农学院;天津大学
关键词:
生菜;子叶;组织培养;植物激素;全息现象
摘  要:
对生菜子叶在含有不同激素浓度的培养基上进行了诱导、分化及成苗的研究,结果表明:在MS+0.5mg/LIAA+1.2mg/L6-BA的培养基上能够实现较高的不定芽再生,不定芽在1/2MS培养基上能健康成苗。同时以生菜子叶不同部位的切块为外植体,用MS+0.5mg/LIAA+1.2mg/L6-BA的培养基进行不定芽诱导试验,发现生菜子叶不同部位的切块在离体培养时存在着全息现象。
译  名:
Research on Tissue culture and the Holographic Phenomenon of Lactuca sativa var.capitata L.
作  者:
SONG Ying-jin1, JI Jing1,LIU Hai-xue2, WANG Gang1(1 Agriculture and Biology Engineering College , Tianjin University , 30072 ; 2 Tianjin Agricultural University, 300384)
关键词:
Lactuca sativa var. capitata L.;Cotyledon;Tissue culture; Phytohormone;The holographic phenomenon
摘  要:
The factors that influence callus induction , shoot differentiation and growth of Lactuca sativa var. capitata L. Cotyledon were studied and an efficient regeneration system was established . Shoot regeneration were better achieved from the medium of MS + 0.5mg·L-1IAA +1.2mg·L-16-BA using different part of cotyledon explant. The shoots could grow up well from the medium of 1/ 2MS without addition of hormone . the holographic phenomenon can be seen using different part of cotyledon explant.

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