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Position: Home > Articles > Studying on Somatic Embryo Induction of Fortunella japonica Fujian Journal of Agricultural Sciences 2014 (5) 454-460

金桔体细胞胚胎诱导的研究

作  者:
黄靖;陈晓静
单  位:
福建农业职业技术学院园艺园林系;福建农林大学园艺学院
关键词:
金桔;组织培养;体细胞胚胎
摘  要:
采用金桔无菌苗叶片为外植体,在单因素试验的基础上,通过正交试验研究不同生长调节剂对金桔胚性愈伤组织诱导的影响。结果表明:MS+2,4-D 2.0mg·L-1+BA 0.5mg·L-1处理最适宜金桔胚性愈伤组织诱导,其中淡黄色愈伤组织团块胚胎发生频率较高;在附加BA 0.05mg·L-1的MS培养基上淡黄色愈伤组织块可形成胚状体;胚状体试管苗在1/2MS+NAA0.1mg·L-1培养基上,生根率可达80%,移栽成活率达70%
译  名:
Studying on Somatic Embryo Induction of Fortunella japonica
作  者:
HUANG Jing;CHEN Xiao-jing;Fujian Agricultural Vocational and Technical College Department of Horticulture and Lanscape;College of Horticulture,Fujian Agriculture and Forestry University;
关键词:
Fortunella japonica;;tissue culture;;somatic embryo
摘  要:
Based on single factorial experiment,the sterile seedlings of Fortunella japonica were used as explants for studying the effects of various growth regulators on inducing embryogeny with orthogonal test.The results showed:the MS+2,4-D 2.0mg·L-1+BA 0.5mg·L-1 was the best culture medium for inducing embryonic callus.Sucrose at MS culture medium with BA 0.05mg·L-1 gave a maximum embryogenesis from the light yellow callus tissue of leaf.Planting somatic embryos on 1/2MS culture medium with NAA0.1mg·L-1,the rooting rate was 80%,the survive rate was 70%.

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