作 者：
叶央芳;严小军;黄晓春;陈烨;陈海敏;朱世华
摘 要：
从青海盐湖底泥中分离出45株嗜盐微生物.其中,丝状真菌F16的抗菌和抗肿瘤活性最强,其发酵液的乙酸乙酯粗提物能抑制4种细菌的生长,尤其对大肠杆菌的抑制作用最强,具有较强的细胞毒活性,当粗提物浓度为50μg.ml-1时对肝癌细胞BEL7402的抑制率可达76.91%.F16菌株的最适生长温度为15℃;培养基盐度升高,对F16的抑制性增强,当盐度超过15%时,F16不生长;在pH值为5～9范围内,F16生长良好.
译 名：
Halophilous microbial groups in saline lake of Qinghai and the growth characteristics and anti-microbial and anti-tumor activities of F16.
作 者：
YE Yangfang,YAN Xiaojun,HUANG Xiaochun,CHEN Ye,CHEN Haimin,ZHU Shihua(College of Life Science and Biotechnology,Ningbo University,Ningbo 315211,China)
关键词：
Halophilous microorganism,Anti-microorganism,Anti-tumor,Saline lake
摘 要：
A total of forty-five halophilous microorganisms were isolated from the sediment of saline lake in Qinghai Province,among which,filamentous fungus F16 showed the highest activity of anti-microorganism and anti-tumor.The ethyl acetate extract of F16 culture filtrate showed a strong cytotoxicity,and could inhibit the growth of four kinds of bacteria,especially Escherichia coli.When the concentration of the crude extract was 50 μg·ml-1,the inhibition rate to liver cancer cell BEL7402 reached 76.91%.The optimal temperature for F16 growth was 15 ℃,and the increase of salt concentration in media would inhibit its growth.When the concentration of salt surpassed 15%,F16 could not survive.F16 grew well when the pH value ranged from 5 to 9.
