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Position: Home > Articles > Effects of Maduramicin Ammonium on Transcriptome and Ultrastructure of Myoblasts Chinese Journal of Veterinary Drug 2024 (6) 40-46

马度米星铵对成肌细胞转录组和超微结构的影响

作者：

陈新;刘畅;张雨梅

单位：

;扬州大学兽医学院兽医药理与毒理学实验室

关键词：

马度米星铵;转录组;空泡;超微结构;成肌细胞

摘要：

探究马度米星铵对成肌细胞基因表达和超微结构的影响.马度米星铵处理C2C12细胞后,于细胞实时分析仪中记录细胞生长情况;利用Illumina HiSeq平台进行转录组测序,筛选差异表达基因并进行基因本体富集分析;显微镜下观察细胞空泡情况并拍照;透射电镜下观察拍照细胞超微结构.结果表明,马度米星铵抑制C2C12细胞生长,呈浓度和时间相关性;1μM马度米星铵处理细胞8 h后,差异表达基因共2450个,其中下调基因为1411个,上调基因1039个;基因本体分析发现,差异表达基因显著富集到空泡、DNA修复、转移酶活性正调控等;细胞内空泡显著增加;1μM马度米星铵处理细胞24 h后,细胞器明显肿胀、线粒体嵴结构髓样变、粗面内质网表面附着核糖体脱颗粒.由此可知,马度米星铵体外可诱导C2C12细胞空泡化,破坏细胞器结构,抑制细胞生长,从而导致其骨骼肌成肌细胞毒性作用,干预细胞空泡化是预防和治疗马度米星铵中毒的重要途径.

译名：

Effects of Maduramicin Ammonium on Transcriptome and Ultrastructure of Myoblasts

单位：

Laboratory of Veterinary Pharmacology and Toxicology,College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009,China

关键词：

maduramicin ammonium%transcriptome%vacuole%ultrastructure%myoblasts

摘要：

This study was set to investigate the effect of maduramicin ammonium on gene expression and ultrastructure in myoblasts.C2C12 cells were treated with maduramicin ammonium,the cell growth was recorded in the cell real-time analyzer;transcriptome sequencing was performed using the Illumina HiSeq platform to screen differentially expressed genes and perform gene ontology enrichment analysis;the vacuoles were observed and photographed under a microscope;the ultrastructure were observed and photographed under a transmission electron microscope.The results showed that maduramicin ammonium inhibited the growth of C2C12 cells in a concentration-and time-dependent manner.After treatment with 1μM maduramicin ammonium for 8 h,there were 2450 differentially expressed genes,including 1411 down-regulated genes and 1039 up-regulated genes.gene ontology analysis showed that the differentially expressed genes were significantly enriched in vacuole,DNA repair,positive regulation of transferase activity,etc.Intracellular vacuoles increased significantly after treatment with 1μM maduramicin ammonium for 8 h.After treatment with 1μM maduramicin ammonium for 24 h,the organelles were obviously swollen,the mitochondrial crista structure was medullated,and ribosome degranulation was attached to the surface of the rough endoplasmic reticulum.The results suggested that maduramicin ammonium induced vacuolation of C2C12 cells and destroyed the structures of organelle,resulting in cell growth inhibition and its toxicity to myoblasts.This study reveals that intervening cell vacuolization is an important means to prevent and treat maduramicin ammonium poisoning.

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马度米星铵对成肌细胞转录组和超微结构的影响

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