关键词：
正番茄斑萎病毒属;朱顶红褪绿环斑病毒;细角带蓟马;荧光定量PCR
摘 要：
【目的】朱顶红褪绿环斑病毒(Hippeastrum chlorotic ringspot virus,HCRV)危害严重,细角带蓟马(Taeniothrips eucharii)是其主要的传播媒介。明确HCRV病毒粒子在细角带蓟马体内的增值规律,为HCRV防控提供理论依据。【方法】应用实时荧光定量PCR技术(qRT-PCR),结合HCRV N基因和细角带蓟马β-actin基因特异性引物,选用SYBR Green方法对获毒后不同虫龄细角带蓟马体内的HCRV含量进行定量分析。【结果】所建立的实时荧光定量方法可以准确特异地对细角带蓟马中的HCRV进行相对定量,发现1龄若虫的HCRV核酸含量最低,1龄若虫、2龄若虫及蛹中的病毒含量没有统计学差异,成虫的HCRV核酸含量最高。【结论】细角带蓟马在获毒后,体内HCRV核酸含量随着细角带蓟马的发育而增加。
译 名：
Proliferative Rule of Hippeastrum chlorotic ringspotvirus in Taeniothrips eucharii
作 者：
WU Jiaqi;XU Ye;LI Yongzhong;LIU Yating;College of Agronomy and Biotechnology,Yunnan Agricultural University;College of Plant Protection,Yunnan Agricultural University;College of Tobacco Science,Yunnan Agricultural University;
关键词：
Orthotospovirus;;Hippeastrum chlorotic ringspot virus (HCRV);;thrips Taeniothrips eucharii;;transmission properties
摘 要：
[Purpose] Hippeastrum chlorotic ringspot virus(HCRV) can cause serious damage and is transmited by Taeniothrips eucharii. The aim of this study was to provide the theoretical evidence for controlling HCRV. [ Method] In this study, SYBR Green method of real-time fluorescent quantitative PCR(qRT-PCR) was used, combined with the specific primers for HCRV N gene and β-actin gene of T. eucharii for quantitative analysis of HCRV content in different treatments. [Reault] This method could accurately and specifically quantify the HCRV virus in T. eucharii. quantitative of HCRV RNA. The concentrate of HCRV was the lowest in 1~(st) larvae. There was no statistical difference in 1~(st) larva, 2~(nd) larva and pura. And the concentrate of HCRV was the highest in adults.HCRV was growing gradually after the 1~(st) larvae acquire HCRV. [Conclusion] This indicated that T. eucharii transmitted HCRV as a persistent-propagative manner.
