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当前位置: 首页 > 文章 > 浙江省蚕区桑树“凋萎”症状病因诊断 蚕业科学 2007,33 (4) 12-17
Position: Home > Articles > The Diagnosis of the Pathogen of Mulberry Bacterial Wilt in Zhejiang Province Science of Sericulture 2007,33 (4) 12-17

浙江省蚕区桑树“凋萎”症状病因诊断

作  者：
吕志强;王汉荣;周勤;王连平;方丽;茹水江;林天宝;叶伟清;朱燕
单  位：
浙江省农业科学院蚕桑研究所;浙江省桐庐县农技推广中心;浙江省农业厅经作局;浙江省农业科学院植物保护与微生物研究所
关键词：
桑树;凋萎症状;细菌性青枯病;雷尔氏菌
摘  要：
从浙江省临安、桐庐等地桑园中的"凋萎"桑树根、茎部获得151个细菌分离物样品。经离体水培接种试验表明,这些分离物均可引起桑树"凋萎"症状,其形态、大小、染色反应、培养性状与对照番茄青枯菌(Ralstonia so-lanacearum)相近;PCR鉴定其中125个分离物为雷尔氏菌(Ralstonia solanacearum)。结合田间发生症状,将桑树"凋萎"症状诊断为细菌性青枯病。从125个雷尔氏菌株中选择16个典型菌株进行研究,根据生理小种划分标准,这些菌株均为雷尔氏菌的生理小种1,其中75%为青枯菌的生化型Ⅴ、25%为青枯菌的生化型Ⅲ。
译  名：
The Diagnosis of the Pathogen of Mulberry Bacterial Wilt in Zhejiang Province
作  者：
Lü Zhi-Qiang1 WANG Hang-Rong2 ZHOU Qin3 WANG Lian-Ping2 FANG Li2 RU Shui-Jiang2 LIN Tian-Bao1 YE Wei-Qing4 ZHU Yan1(1Sericultural Research Institute,Zhejiang Academy of Agricaltural Sciences,Hangzhou 310021,China;2 Institute of Plant Protection and Microbiology,Zhejiang Academy of Agricaltural Sciences,Hangzhou 310021,China;3Industrial Crop Bureau,Agriculture Department of Zhejiang Province,Hangzhou 310020,China;4Tonglu County Agricultural Extension Centre of Zhejiang Province,Tonglu Zhejiang 311500,China)
关键词：
Mulberry;Wilt character;Bacterial wilt;Ralstonia solanacearum
摘  要：
One hundred and fifty-one bacterial strains were isolated from the roots and stems of wizened mulberry in Ling'an and Tonglu counties of Zhejiang Province.Pathogenicity test results show that all of the strains can induce the mulberry wilt,and the shape,size,dye-reaction and culture characteristics of the mulberry wilt were similar to those of the tomato bacterial wilt.Based on PCR results,125 bacterial strains were identified as Ralstonia solanacearum.Based on the symptom in farm,the mulberry wilt were diagnosed as bacterial wilt.Among them,16 typical strains were also classified as the physiological race 1 by Haywind's classification criterion,and 75% of which were Ralstonia solanacearum biovar Ⅴ,25% were Ralstonia solanacearum biovar Ⅲ.

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