Establishment of Detection Methods for Betamethasone in the 4 Kinds of Veterinary Injections by UPLC-PDA

HAN Chun-Xiao;ZHANG Yue;LI Hong-wei;XU Bin;LI Xin-nan;Liaoning Province Animal Feed Quality and Safety of Veterinary Testing Centers;School of Traditional Chinese Materia Medica ,Shenyang Pharmaceutical University;

UPLC;;bupleurum injection;;vitamin C injection;;lincomycin hydrochloride injection;;asragulus polysacharin injection;;betamethasone

A method for the determination of betamethasone in bupleurum injection,lincomycin hydrochloride injection,vitamin C injection,and asragulus polysacharin injection was developed by UPLC-PDA. It was tested with the Waters ACQUITY UPLCBEH C18 column( 2. 1 mm × 50 mm,1. 7 μm),using 0. 75% tri-ethylamine phosphatic liquor/solution( p H = 3. 0)-methyl alcohol-acetonitrile-( 50 ∶ 45 ∶ 5) as the mobile phase; The flow rate was 0. 3 m L/min; The detection wavelength was set at 242 nm and simultaneously collected the spectrum at wavelengthof 190 ~ 400 nm. The column temperature had been remained in 25 ℃. The injection volume was10 μL. The linear range of betamethasone was 20. 0 ~ 180. 0 mg·L-1( R2= 0. 9997,n = 7); The mean recovery for betamethasone in bupleurum injection,lincomycin hydrochloride injection,vitamin C injection and asragulus polysacharin injection were 103. 5%( RSD = 0. 2%),101. 2%( RSD = 0. 8%),100. 4%( RSD = 1. 7%),99. 2%( RSD = 1. 7%). This method is accurate,easy and fast,and provides a reliable way for detecting betamethasone in bupleurum injection and another three kinds of veterinary injections.