Position: Home > Articles > Metabolites Profile Analysis of Fermented Milk with Lactobacillus plantarum P-8 Based on Ultra-performance Liquid Chromatography-quadrupole-time of Flight Mass Spectrometry(UPLC-Q-TOF-MS)
Science and Technology of Food Industry
2019
(11)
152-160
基于UPLC-Q-TOF-MS的植物乳杆菌P-8发酵乳代谢物轮廓分析
作 者：
王越男;米智慧;李常坤;潘琳;孙志宏;孙天松
单 位：
内蒙古农业大学乳品生物技术与工程教育部重点实验室农业部奶制品加工重点实验室
关键词：
代谢物轮廓;UPLC-Q-TOF MS;发酵乳;植物乳杆菌P-8;代谢组学
摘 要：
代谢组学是对生物体受外部刺激所产生的小分子代谢产物的变化或其随时间的变化进行研究的一门学科。本实验采用超高效液相色谱和四级杆飞行时间串联质谱技术结合PCA分析了植物乳杆菌P-8发酵乳的代谢物图谱。结果表明:通过代谢组学的分析方法共鉴定152个代谢物,差异代谢物主要为有机酸16种(琥珀酸、柠檬酸和顺乌头酸等)、多肽20种(Thr-Val、Asn-Pro、Val-Asn、Asn-Thr、Asn-Leu、Trp-Ala和Try-Ser等)、游离氨基酸12种(半胱氨酸、亮氨酸、赖氨酸、精氨酸和酪氨酸等)和核酸代谢物8种(7H-Purine、Cytidine和Pyrimidine等)。因此,利用此方法能够对发酵乳代谢物进行鉴定和分析,并作为其潜在的生物标记物。
译 名：
Metabolites Profile Analysis of Fermented Milk with Lactobacillus plantarum P-8 Based on Ultra-performance Liquid Chromatography-quadrupole-time of Flight Mass Spectrometry(UPLC-Q-TOF-MS)
作 者：
WANG Yue-nan;MI Zhi-hui;LI Chang-kun;PAN Lin;SUN Zhi-hong;SUN Tian-song;Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture,Inner Mongolia Agricultural University;
单 位：
WANG Yue-nan%MI Zhi-hui%LI Chang-kun%PAN Lin%SUN Zhi-hong%SUN Tian-song%Key Laboratory of Dairy Biotechnology and Engineering,Ministry of Education,Key Laboratory of Dairy Products Processing,Ministry of Agriculture,Inner Mongolia Agricultural University
关键词：
metabolites profile;;UPLC-Q-TOF-MS;;fermented milk;;Lactobacillus plantarum P-8;;metabolomics
摘 要：
Metabolomics is a subject that studies variations or temporal variations of micromolecule metabolites generated by organisms due to exterior stimulus. Metabolites profile of Lactobacillus plantarum P-8 fermented milk was analyzed by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS)combined principal component analysis(PCA). The results showed that 152 metabolites were determined as the major compounds contributing to the discrimination of fermented milk. The differential metabolites were mainly 16 organic acids(succinic acid,citric acid and homo-cis-aconitate etc.)and 20 peptides(Thr-Val,Asn-Pro,Val-Asn,Asn-Thr,Asn-Leu,Trp-Ala and Try-Ser etc.),12 free amino acids(cysteine,leucine,lysine,arginine and tyrosine,etc.)and 8 nucleic acid metabolites(7 H-purine,cytidine and pyrimidine,etc.). Therefore,metabolomic methods could be used to identify and analyze different metabolites in fermented milk,which could serve as potential biomarkers.
导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0
所属期刊
